Reaction chamber having pre-stored reagents
Disclosed are preloaded analysis modules comprising reagents disposed within a barrier material capable of liberating the reagents, at a time advantageous to a reaction scheme, when exposed to certain activation conditions or reagents. Also disclosed are related methods for analyzing samples with such modules.
1. A method for analyzing a sample using a preloaded analysis module, the preloaded analysis module defining a chamber having an inlet and an outlet, the chamber also including one or more recesses in a top surface of the chamber, the preloaded analysis module comprising one or more reagents disposed within a barrier material, the one or more reagents and the barrier material being disposed within the chamber, the one or more reagents being essentially inert to the barrier material, the barrier material being inert to aqueous media, and the barrier material being capable of releasing the one or more reagents upon activation, the method comprising:
introducing a sample into the chamber through the inlet;
activating the barrier material so as to release one or more reagents, wherein the chamber and the barrier material are configured such that when the barrier material is activated, the barrier material floats upward and the one or more recesses traps the barrier material to as to prevent the barrier material from floating or flowing into the outlet;
reacting the one or more reagents react with the sample; and
after the reacting step, removing the sample from the chamber through the outlet.
2. The method of claim 1 , wherein the chamber is disposed on a substrate.
3. The method of claim 1 , wherein the introducing step comprises flowing, dropping, spraying, or any combination thereof of the sample into the chamber through the inlet.
4. The method of claim 1 , wherein the step of introducing the sample into the chamber includes introducing the sample disposed in an aqueous medium.
5. The method of claim 1 , wherein the activating step comprises heating, irradiating, illuminating, magnetizing, electrifying, sonicating, or any combination thereof.
6. The method of claim 1 , wherein the reacting step comprises contacting, polymerizing, binding, neutralizing, acidifying, lysing, de-polymerizing, transfecting, infecting, de-acidifying, annealing, labeling, tagging, binding, amplifying, or any combination thereof.
7. The method of claim 1 , wherein the reacting step comprises a polymerase chain reaction.
8. The method of claim 6 , further comprising heating at least a portion of the chamber, cooling at least a portion of the chamber, applying an electric current to at least a portion of the chamber, applying a magnetic field to at least a portion of the chamber, introducing one or more additional fluids into the chamber, introducing one or more additional reagents into the chamber, or any combination thereof.
9. The method of claim 1 , further comprising a step of monitoring progress of the reacting step.
10. The method of claim 9 , wherein the monitoring step comprises monitoring production of one or more products of the reacting step.
11. The method of claim 10 , wherein the monitoring step comprises visually inspecting, monitoring an electrical signal, monitoring a chemical property, or any combination thereof.