Compositions and methods for the therapy and diagnosis of influenza
The present invention provides compositions, vaccines, and methods for diagnosing, treating, and preventing influenza infection using a combination of antibodies raised against the influenza hemagglutinin and the matrix 2 ectodomain polypeptides.
1. A composition comprising:
(a) an isolated human monoclonal antibody, wherein said antibody comprises a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 771 and a light chain variable region comprising the amino acid sequence of SEQ ID NO: 778; and
(b) an isolated human monoclonal antibody, wherein said antibody comprises a VH CDR1 region comprising the amino acid sequence of NYYWS (SEQ ID NO: 72); a VH CDR2region comprising the amino acid sequence of FIYYGGNTKYNPSLKS (SEQ ID NO: 74); a VH CDR3 region comprising the amino acid sequence of ASCSGGYCILD (SEQ ID NO: 76); a VL CDR1 region comprising the amino acid sequence of RASQNIYKYLN (SEQ ID NO: 59); a VL CDR2 region comprising the amino acid sequence of AASGLQS (SEQ ID NO: 61); a VL CDR3region comprising the amino acid sequence of and QQSYSPPLT (SEQ ID NO: 63),
wherein at least one of the antibodies is directly conjugated to either a detectable label or therapeutic agent.
2. A passive immunization composition comprising the composition of claim 1 .
3. A pharmaceutical composition comprising the composition of claim 1 and a pharmaceutical carrier.