Fluorescent color markers
View Patent ↗The invention provides a yeast-enhanced red fluorescent protein. In an embodiment of the invention, the yeast-enhanced red fluorescent protein is monomeric and is expressible in Candida albicans . The invention also provides a novel visible color marker for plasmid expression in yeast, particularly Saccharomyces cerevisiae and Candida albicans.
1. An isolated nucleic acid comprising the nucleic acid sequence of SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, or SEQ ID NO:9, or the full length complement thereof.
2. The isolated nucleic acid molecule of claim 1 , which further comprises an expression control sequence.
3. A host cell comprising the nucleic acid of claim 1 .
4. The host cell of claim 3 , wherein the host cell is E. coli.
5. The host cell of claim 3 , wherein the host cell is S. cerevisiae.
6. The host cell of claim 3 , wherein the host cell is C. albicans.
7. A vector comprising the nucleic acid of claim 1 .
8. A host cell comprising the vector of claim 7 .
9. The host cell of claim 8 , wherein the host cell is E. coli.
10. The host cell of claim 8 , wherein the host cell is S. cerevisiae.
11. A kit comprising the vector of claim 7 .
12. A kit comprising the host cell of claim 3 .
13. The kit of claim 12 , wherein the host cell is C. albicans.
14. The kit of claim 12 , wherein the host cell is S. cerevisae.
15. The host cell of claim 8 , wherein the host cell is C. albicans.