Biokinetics of fast-clearing polypeptides
Provided herein are methods of reducing liver uptake in vivo of a polypeptide that specifically binds to a target comprising: (a) providing a polypeptide that specifically binds to a target; and (b) substituting at least one basic or at least one neutral amino acid residue of the native polypeptide from step (a) with an acidic amino acid residue to produce a modified polypeptide, wherein the modified polypeptide demonstrates an isoelectric point at least 0.05-0.1 pH points less than the isoelectric point of the native polypeptide. Also provided are polypeptides made using the inventive methods as well as imaging techniques that employ the methods and agents.
1. A method of reducing liver or cardiovascular uptake in Vivo in subject in need thereof, of a native polypeptide comprising the amino acid SEQ. ID No. 5 that specifically binds to a PDGF-Rβ of the subject comprising:
(i) generating a labeled modified polypeptide in vitro by:
(a) providing the native polypeptide comprising the amino acid sequence of SEQ. ID NO. 5 that specifically binds to the PDGF-Rβ;
(b) substituting R20, L21, K22, A25, A29, K36, S44, and R47 residues of the native polypeptide from step (a) with L, S, D, Q, S, S, K, and I residues respectively to produce a modified polypeptide comprising the amino acid sequence of SEQ. ID NO. 2, wherein the modified polypeptide (SEQ. ID NO. 2)demonstrates an isoelectric point at least 0.05-0.1 pH points less than the isoelectric point of the native polypeptide;
(c) appending a signal generator to the modified polypeptide to form a labeled modified polypeptide comprising the amino acid sequence of SEQ. ID NO. 2; and
(ii) introducing the labeled modified polypeptide of comprising the amino acid sequence of SEQ. ID NO. 2 into the subject to specifically bind to the PDGF-Rβ in vivo.
2. The method of claim 1 , wherein the signal generator is selected from radioactive, fluorescent, magnetic, radioopaque, luminescent, phosphorescent, isotopic, or ultrasound opaque signal generator.
3. The method of claim 2 , wherein the signal generator comprises 99 mTc or 18 F.
4. The method of claim 1 , further comprising a targeting moiety appended to the modified polypeptide.