Humanized IL-7 rodents
Genetically modified non-human animals comprising a human or humanized interleukin-7 (IL-7) gene. Cells, embryos, and non-human animals comprising a human or humanized IL-7 gene. Rodents that express human or humanized IL-7 protein. Genetically modified mice that comprise a human or humanized IL-7-encoding gene in their germline, wherein the human or humanized IL-7-encoding gene is under control of endogenous mouse IL-7 regulatory sequences.
1. A mouse whose genome comprises a replacement of at least mouse IL-7 exons 2, 3, 4, and 5 with a human genomic segment comprising human IL-7 exons 2, 3, 4, 5, and 6 to form a human or humanized IL-7-encoding gene, wherein the replacement is at an endogenous mouse IL-7 locus, wherein the human or humanized IL-7-encoding gene is under control of endogenous mouse IL-7 regulatory elements, and wherein the mouse comprises human or humanized IL-7 in serum at a level that is about the same level of endogenous mouse IL-7 in serum of an age matched wild-type mouse.
2. The mouse of claim 1 , wherein the level is about 2.4 to about 3.2 pg/mL.
3. The mouse of claim 1 , wherein said replacement forms a human IL-7-encoding gene, and wherein the mouse comprises human IL-7 in serum at a level that is about the same level of endogenous mouse IL-7 in serum of an aged-matched wild-type mouse.
4. A method for making a mouse with a human or humanized IL-7-encoding gene, comprising modifying the genome of a mouse by replacing at the endogenous mouse IL-7 gene locus at least mouse exons 2, 3, 4 and 5 of the endogenous mouse IL-7-encoding gene with a human genomic segment comprising human IL-7 exons 2, 3, 4, 5, and 6 to form a human or humanized IL-7-encoding gene, wherein the human or humanized IL-7-encoding gene is under control of endogenous mouse IL-7 regulatory sequences, and wherein the mouse comprises human or humanized IL-7 in serum at a level that is about the same level of endogenous mouse IL-7 in serum of an aged-matched wild-type mouse.
5. The method of claim 4 , wherein the level is about 2.4 to about 3.2 pg/mL.
6. The method of claim 4 , wherein said human or humanized IL-7 gene encodes human IL-7, and wherein the mouse comprises human IL-7 in serum at a level that is about the same level of endogenous mouse IL-7 in serum of an aged-matched wild-type mouse.