Production of volatile dienes by enzymatic dehydration of light alkenols
Described is a method for generating conjugated dienes through a biological process. More specifically, the application describes a method for producing conjugated dienes (for example butadiene, isoprene or dimethylbutadiene) from light alkenols via enzymatic dehydration, in particular by making use of an alkenol dehydratase.
1. A method for producing isoprene comprising enzymatically converting a C 5 H 10 O alcohol to isoprene, wherein said C 5 H 10 O alcohol is 3-methylbut-3-en-1-ol, 2-methylbut-2-en-1-ol, 3-methylbut-3-en-2-ol, or 2-methylbut-3-en-1-ol, by an enzyme at least 70% identical to the amino acid sequence of SEQ ID NO:1, wherein said enzyme has alkenol dehydratase activity.
2. The method of claim 1 , wherein the C 5 H 10 O alcohol is 3-methylbut-3-en-1-ol.
3. The method of claim 1 , wherein the C 5 H 10 O alcohol is 2-methylbut-2-en-1-ol.
4. The method of claim 1 , wherein the C 5 H 10 O alcohol is 3-methylbut-3-en-2-ol.
5. The method of claim 1 , wherein the C 5 H 10 O alcohol is 2-methylbut-3-en-1-ol.
6. The method of claim 1 , wherein said method is carried out in vitro.
7. The method of claim 1 , wherein said method is carried out in a microorganism.
8. The method of claim 1 , wherein the enzyme is at least 80% identical to the amino acid sequence of SEQ ID NO:1.
9. The method of claim 1 , wherein the enzyme is at least 90% identical to the amino acid sequence of SEQ ID NO:1.
10. The method of claim 9 , wherein said method is carried out in vitro.
11. The method of claim 9 , wherein said method is carried out in a microorganism.
12. The method of claim 1 , wherein the enzyme is at least 95% identical to the amino acid sequence of SEQ ID NO:1.
13. The method of claim 1 , wherein the enzyme is the amino acid sequence of SEQ ID NO:1.
14. A composition comprising a microorganism which comprises an enzyme at least 70% identical to the amino acid sequence of SEQ ID NO:1, wherein said enzyme has alkenol dehydratase activity, and a C 5 H 10 O alcohol, wherein said C 5 H 10 O alcohol is 3-methylbut-3-en-1-ol, 2-methylbut-2-en-1-ol, 3-methylbut-3-en-2-ol, or 2-methylbut-3-en-1-ol.
15. The composition of claim 14 , wherein the C 5 H 10 O alcohol is 3-methylbut-3-en-1-ol.
16. The composition of claim 14 , wherein the C 5 H 10 O alcohol is 2-methylbut-2-en-1-ol.
17. The composition of claim 14 , wherein the C 5 H 10 O alcohol is 3-methylbut-3-en-2-ol.
18. The composition of claim 14 , wherein the C 5 H 10 O alcohol is 2-methylbut-3-en-1-ol.
19. The composition of claim 14 , wherein the enzyme is at least 80% identical to the amino acid sequence of SEQ ID NO:1.
20. The composition of claim 14 , wherein the enzyme is at least 90% identical to the amino acid sequence of SEQ ID NO:1.
21. The composition of claim 14 , wherein the enzyme is at least 95% identical to the amino acid sequence of SEQ ID NO:1.
22. The composition of claim 14 , wherein the enzyme is the amino acid sequence of SEQ ID NO:1.
23. A composition comprising an enzyme at least 70% identical to the amino acid sequence of SEQ ID NO:1, wherein said enzyme has alkenol dehydratase activity, and an C 5 H 10 O alcohol, wherein said C 5 H 10 O alcohol is 3-methylbut-3-en-1-ol, 2-methylbut-2-en-1-ol, 3-methylbut-3-en-2-ol, or 2-methylbut-3-en-1-ol.
24. The composition of claim 23 , wherein the C 5 H 10 O alcohol is 3-methylbut-3-en-1-ol.
25. The composition of claim 23 , wherein the C 5 H 10 O alcohol is 2-methylbut-2-en-1-ol.
26. The composition of claim 23 , wherein the C 5 H 10 O alcohol is 3-methylbut-3-en-2-ol.
27. The composition of claim 23 , wherein the enzyme is at least 80% identical to the amino acid sequence of SEQ ID NO:1.
28. The composition of claim 23 , wherein the enzyme is at least 90% identical to the amino acid sequence of SEQ ID NO:1.
29. The composition of claim 23 , wherein the enzyme is at least 95% identical to the amino acid sequence of SEQ ID NO:1.
30. The composition of claim 23 , wherein the enzyme is the amino acid sequence of SEQ ID NO:1.