B7-H1 Antibodies
View Patent ↗The invention provides novel polypeptides useful for co-stimulating T cells, isolated nucleic acid molecules encoding them, vectors containing the nucleic acid molecules, and cells containing the vectors. Also included are methods of making and using these co-stimulatory polypeptides.
1. An isolated antibody that binds specifically to a B7-H1 polypeptide which comprises
(a) amino acids having the sequence set forth in SEQ ID NO:1; or
(b) amino acids that are encoded by a polynucleotide comprising nucleotides having the sequence set forth in SEQ ID NO:2.
2. The isolated antibody of claim 1 , which is a monoclonal antibody.
3. The isolated antibody of claim 1 , which binds to the polypeptide comprising amino acids having the sequence set forth in SEQ ID NO:1.
4. An antibody fragment that specifically binds to a B7-H1 polypeptide which comprises
(a) amino acids having the sequence set forth in SEQ ID NO:1; or
(b) amino acids that are encoded by a polynucleotide comprising nucleotides having the sequence set forth in SEQ ID NO:2.
5. The antibody fragment of claim 4 , which is an F(ab) 2 fragment, an Fab fragment or a single-chain Fv fragment (scFv) and which specifically binds to the polypeptide of (a) or (b).
6. The monoclonal antibody of claim 2 , which is a chimeric or a humanized monoclonal antibody.
7. A hybridoma which produces a monoclonal antibody according to claim 2 .
8. The isolated antibody according to claim 1 , which binds to a polypeptide comprising amino acids having a sequence from amino acid residue 23 to amino acid residue 290 of SEQ ID NO:1; or
a polypeptide comprising one or more conservative substitutions in SEQ ID NO:1.
9. The isolated antibody according to claim 1 , which binds to a polypeptide comprising amino acids having a sequence from about amino acid residue 20 to amino acid residue 290 of SEQ ID NO:1.
10. The isolated antibody according to claim 1 , which binds to a polypeptide comprising amino acids having a sequence from amino acid residue 23 to amino acid residue 290 of SEQ ID NO:1.
11. An isolated antibody which selectively binds to a B7-H1 polypeptide which
(a) comprises amino acids having a sequence from amino acid residue 23 to amino acid residue 239 of SEQ ID NO:1.
12. An isolated antibody or an antigen binding fragment thereof which selectively binds to the polypeptide of SEQ ID NO:1.
13. The isolated antibody according to claim 12 which is a polyclonal, a monoclonal, a chimeric, a human, a humanized antibody, or an antigen binding antibody fragment thereof.
14. The isolated antibody according to claim 12 which is an Fab antibody fragment, an F(ab′)2 antibody fragment, a single chain antibody fragment, an scFv antibody fragment, or an antigen-binding fragment thereof.
15. The isolated antibody according to claim 12 which is attached to a solid substrate.
16. The isolated antibody of claim 12 , which inhibits the co-stimulatory activity of said B7-H1 polypeptide of SEQ ID NO:1.
17. The isolated antibody of claim 12 , which inhibits the co-stimulatory activity of said B7-H1 polypeptide of SEQ ID NO:1 on a T-cell.
18. An isolated cell that is capable of expressing the antibody of claim 1 .
19. A composition comprising the antibody of claim 1 and a pharmaceutically acceptable excipient.
20. The composition of claim 19 , wherein said antibody is a polyclonal antibody, a monoclonal antibody, a chimeric antibody, a human antibody, or a humanized antibody.
21. The composition of claim 19 , wherein said antibody inhibits the co-stimulatory activity of said B7-H1 polypeptide of SEQ ID NO:1.
22. A method for detecting the presence of a B7-H1 polypeptide in a sample comprising:
(a) contacting the sample with the isolated antibody of claim 1 ; and
(b) determining the binding of said antibody to the polypeptide in the sample to thereby detect the presence of a B7-H1 polypeptide in the sample.
23. An isolated antibody or an antigen binding fragment thereof which specifically binds to a polypeptide consisting of amino acids having the sequence set forth in SEQ ID NO:1.
24. A method of making an antibody, or an antigen binding fragment thereof comprising:
providing a polypeptide which is (a) a polypeptide comprising amino acids having the sequence set forth in SEQ ID NO:1; or (b) a polypeptide which is encoded by a polynucleotide comprising nucleotides having the sequence set forth in SEQ ID NO:2 to an immunocompetent vertebrate; and
harvesting from the vertebrate blood or serum comprising an antibody which specifically binds to said polypeptide, or an antigen binding fragment thereof.
25. A composition comprising the antibody fragment according to claim 4 and a pharmaceutically acceptable excipient.
26. The composition according to claim 25 , wherein said antibody fragment is an Fab fragment, an F(ab′)2 fragment, a single chain antibody fragment, an scFv antibody fragment, or an antigen-binding fragment thereof.
27. An antibody or an antigen binding fragment thereof which binds to a soluble B7-H1 polypeptide which is not membrane-bound, wherein said soluble B7-H1 polypeptide comprises amino acid domain(s) having the sequence(s) shown in FIG. 2A (SEQ ID NO:1) such that the polypeptide is soluble.