Method and apparatus for fluid dispersion
A microfluidic method and device for focusing and/or forming discontinuous sections of similar or dissimilar size in a fluid is provided. The device can be fabricated simply from readily-available, inexpensive material using simple techniques.
1. A device comprising:
a microfluidic interconnected region comprising an upstream portion, a downstream portion and a dimensionally-restricted section separating the upstream portion from the downstream portion of the microfluidic interconnected region, the dimensionally-restriction section having a mean cross-sectional dimension that is dimensionally restricted relative to the upstream portion and the downstream portion, the upstream portion comprising a junction of two microfluidic inlet channels each containing a continuous fluid and one microfluidic inlet channel containing a subject fluid, wherein the continuous fluid completely circumferentially surrounds the subject fluid when the continuous fluid and the subject fluid flow through the microfluidic interconnected region.
2. The device of claim 1 , wherein the dimensionally-restricted section comprises a non-valved orifice.
3. The device of claim 1 , wherein the dimensionally-restricted section is formed by at least an extension of a wall defining the interconnected region.
4. The device of claim 1 , wherein the microfluidic interconnected region and the two or more microfluidic channels are part of a single integral unit.
5. The device of claim 1 , wherein the microfluidic interconnected region, the upstream portion, and the downstream portion are each contained within a microfluidic device.
6. The device of claim 1 , wherein the microfluidic interconnected region has a maximum cross-sectional diameter of less than 50 microns.
7. The device of claim 1 , wherein the downstream portion has a largest dimension perpendicular to fluid flow of less than about 1 mm.
8. The device of claim 1 , wherein the subject fluid forms discontinuous sections at the interconnected region surrounded by the continuous fluid, at least some of the discontinuous sections having a maximum dimension of less than 100 microns.
9. The device of claim 1 , wherein the microfluidic inlet channel containing the subject fluid has an outlet upstream of the dimensionally-restricted section.
10. The device of claim 1 , wherein the interconnected region and the microfluidic inlet channel containing the subject fluid comprise a central axis.
11. The device of claim 1 , wherein the continuous phase comprises oil.
12. The device of claim 1 , wherein the subject fluid comprises water.
13. The device of claim 1 , wherein each the subject fluid and the continuous fluid each have a flow rate, and the ratio of the flow rate of the subject fluid to the flow rate of the continuous fluid is less than 1:5.
14. The device of claim 8 , wherein the continuous fluid is immiscible in the subject fluid within the time frame of formation of the discontinuous sections of the subject fluid.
15. The device of claim 8 , wherein at least some of the discontinuous sections have a maximum dimension of less than 80 microns.
16. The device of claim 8 , wherein at least some of the discontinuous sections have a maximum dimension of less than 60 microns.
17. The device of claim 8 , wherein at least some of the discontinuous sections have a maximum dimension of less than 40 microns.
18. The device of claim 8 , wherein at least some of the discontinuous sections have a maximum dimension of less than 20 microns.