Core-shell magnetic particles and related methods
The invention provides core-shell magnetic particles comprising a magnetic core and a functional shell, methods for making same, methods of separation using same, methods for using same, and devices comprising same. The particles and methods of the invention are useful for targeting and removing substances of interest that may be found in complex mixtures.
1. A particle, comprising a plurality of magnetic nanoparticles, and a silicon oxide shell surrounding the plurality of magnetic nanoparticles, wherein the silicon oxide shell integrally comprises a plurality of functionalized alkyl silanes, wherein each functionalized alkyl silane comprises a reactive functional group which is an electrophilic group.
2. The particle of claim 1 , wherein the magnetic nanoparticles comprise magnetite.
3. The particle of claim 1 , wherein the electrophilic group is selected from the group consisting of epoxy, alkyl halide, ester, aldehyde, and ketone.
4. A particle, represented by (MS)-L 1 -X-L 2 -FG 3 , wherein
(MS) is a particle comprising a plurality of magnetic nanoparticles and a silicon oxide shell surrounding the plurality of magnetic nanoparticles;
L l is an alkyl or alkyloxy linker moiety integral to the silicon oxide shell;
L 2 is an alkyl or alkyloxy linker moiety;
X is a covalent linkage, joining L l and L 2 , selected from the group consisting of
FG 3 is a reactive functional group which is an electrophilic group covalently linked to L 2 ; and
R is H or alkyl.
5. The particle of claim 4 , wherein the magnetic nanoparticles comprise magnetite.
6. The particle of claim 4 , wherein the electrophilic group is selected from the group consisting of epoxy, alkyl halide, ester, aldehyde, and ketone.
7. The particle of claim 4 , wherein L 2 -FG 3 is a polymer.
8. A method for producing a particle, comprising
combining (i) an aqueous solution of magnetic nanoparticles, (ii) a reactive silicate, and (iii) a functionalized alkyl silane comprising a first reactive functional group which is an electrophilic group, under conditions permitting formation of a silicon oxide shell surrounding the magnetic nanoparticles and integrally comprising the functionalized alkyl silane.
9. The method of claim 8 , further comprising
linking the functionalized alkyl silane to a molecule comprising a second reactive functional group through a covalent bond formed by reaction of the first reactive functional group with the second reactive functional group, wherein the second reactive functional group is (i) complementary to the first reactive functional group and (ii) a nucleophilic group.
10. A method of separation, comprising
combining a substance of interest, an effective amount of a particle of claim 1 , and a solvent, thereby forming a mixture comprising a complex comprising the substance of interest and the particle; and
applying to the mixture a magnetic field of effective strength to influence mobility of the complex in the mixture.
11. A method of separation, comprising
combining a substance of interest, an effective amount of a particle of claim 4 , and a solvent, thereby forming a mixture comprising a complex comprising the substance of interest and the particle; and
applying to the mixture a magnetic field of effective strength to influence mobility of the complex in the mixture.
12. A method of killing a bacterium, comprising
combining a bacterium, an effective amount of a particle of claim 4 , and a solvent, thereby forming a mixture comprising a complex comprising the bacterium and the particle.
13. The particle of claim 4 , wherein X is