Polymethine compounds and their use as fluorescent labels
The present disclosure relates to new polymethine compounds and their use as fluorescent labels. The compounds may be used as fluorescent labels for nucleotides in nucleic acid sequencing applications.
1. A compound of formula (I) or mesomeric forms thereof:
wherein mCat+ or mAn− is an organic or inorganic positively/negatively charged counterion and m is an integer 0-3;
each of Ra 1 and Ra 2 is independently H, SO 3 − , sulfonamide, halogen, or a further ring fused to an adjacent carbon atom;
Rb is alkyl or alkyl substituted with a carboxy or a sulfonic group or derivatives thereof;
each of Rc 1 and Rc 2 is independently alkyl or substituted alkyl; and
either Rb or one of Rc 1 or Rc 2 contains a linking moiety for further attachment or is linked to a further molecule.
2. A compound according to claim 1 wherein Rc1 or Rc2 is methyl, ethyl, propyl or —(CH 2 ) q SO 3 − where q is 1-6.
3. A compound according to claim 1 wherein one of the Ra groups is a further fused ring forming a structure of formula (II):
wherein Ra 1 is H, SO 3 − , sulphonamide or halogen; and
Rc 1 is alkyl or substituted alkyl.
4. A compound according to claim 1 wherein the linking moiety is attached to Rb.
5. A compound according to claim 1 wherein the compound is attached to a nucleotide or oligonucleotide via Rb.
6. A nucleotide or oligonucleotide labelled with a compound according to claim 1 .
7. A labelled nucleotide or oligonucleotide according to claim 6 wherein the label is attached via substituted alkyl group Rb.
8. A labelled nucleotide or oligonucleotide according to claim 6 wherein the label is attached to the C5 position of a pyrimidine base or the C7 position of a 7-deaza purine base through a linker moiety.
9. A labelled nucleotide or oligonucleotide according to claim 6 , further comprising a 3′ OH blocking group covalently attached to the ribose or deoxyribose sugar of the nucleotide.
10. A kit comprising two or more nucleotides wherein at least one nucleotide is a labelled nucleotide according to claim 6 .
11. A kit according to claim 10 wherein two of the labelled nucleotides are excited using a single laser.
12. A kit according to claim 10 further comprising a second, third, and fourth nucleotide, each labelled with a different compound, wherein each compound has a distinct absorbance maximum and each of the compounds is distinguishable from the other three compounds.
13. A kit according to claim 10 wherein a first of four nucleotides is a labelled nucleotide according to claim 6 and two of the compounds have a distinct absorbance maximum above 600 nm.
14. A method of sequencing comprising:
a) providing a nucleotide according to claim 6 ;
b) incorporating the nucleotide into the complement of an immobilized target polynucleotide; and
c) detecting the nucleotide incorporated in step b).
15. The method according to claim 14 , wherein said detecting is performed on an automated sequencing instrument wherein said automated sequencing instrument comprises two light sources operating at different wavelengths.
16. A method of synthesising a compound according to claim 1 comprising:
a) providing one or more of the following starting material:
or a salt thereof wherein Ra is H, SO3-, sulfonamide, halogen, or a further ring fused to an adjacent carbon atom; and
b) performing a synthetic reaction with said starting material to obtain a compound according to claim 1 .