Recombinant microorganism having butanol production capacity and butanol production method
This invention relates to a recombinant microorganism having improved butanol production capacity and butanol production with the use of such recombinant microorganism with good efficiency. In this invention, the acetoacetyl-CoA synthase gene encoding an enzyme capable of synthesizing acetoacetyl-CoA from malonyl-CoA and acetyl-CoA and a group of genes involved in butanol biosynthesis that enables synthesis of butanol from acetoacetyl-CoA are introduced into a host microorganism.
1. A recombinant microorganism, comprising an acetoacetyl-CoA synthase gene encoding a protein having the amino acid sequence of SEQ ID NO: 1, and a group of genes from Clostridium acetobutvlicum that are involved in butanol biosynthesis that enables synthesis of butanol from acetoacetyl-CoA, wherein the genes are introduced into a host microorganism, wherein the group of genes involved in butanol biosynthesis consists of the β-hydroxybutyryl-CoA dehydrogenase gene, the 3-hydroxybutyryl-CoA dehydratase gene, the butyryl-CoA dehydrogenase gene, the butyraldehyde dehydrogenase gene, and the butanol dehydrogenase gene, wherein the acetoacetyl-CoA synthase gene is a gene from an actinomycete of the genus Streptomyces , and wherein the host microorganism is Escherichia coli.
2. A butanol production method, comprising steps of:
culturing a recombinant microorganism according to claim 1 in a culture medium; and
collecting butanol from the medium.