Systems and methods for monitoring the amplification of DNA
A system and method for amplifying and detecting nucleic acids are disclosed. In one embodiment, the system includes: a microfluidic device comprising a channel for receiving a sample of solution containing real-time PCR reagents; a temperature control system configured to cycle the temperature of the sample; an excitation source for illuminating the sample; a fiber optic probe comprising (i) an optical fiber having a distal end and a proximal end and (ii) a probe head connected to the distal end of the optical fiber and positioned between the distal end of the optical fiber and the channel; and a detector configured to detect emissions exiting the proximal end of the optical fiber.
1. A system for performing real-time PCR, comprising:
a microfluidic device comprising a microfluidic chip comprising at least one channel for receiving a sample of solution containing real-time PCR reagents;
a temperature control system configured to cycle the temperature of the sample; and
an imaging system for detecting emissions from the sample, wherein the imaging system comprises:
an excitation source for illuminating the sample,
at least two fiber optic probes, each optic probe comprising (i) an optical fiber having a distal end and a proximal end and (ii) a probe head connected to the distal end of the optical fiber and positioned between the distal end of the optical fiber and the channel,
a detector configured to detect emissions exiting the proximal end of the optical fiber, wherein the at least two fiber optic probes are fixed along the length of each of the at least one channel; and
a main controller in communication with said temperature control system and said imaging system.
2. The system of claim 1 , wherein said probe head is positioned directly above the channel and is positioned no more than about 10 millimeters from the top of the channel.
3. The system of claim 1 , further comprising a positioning system configured to scan at least one additional fiber optic probes over at least a portion of the channel.
4. The system of claim 2 , further comprising a pump for causing the sample to flow through the channel.
5. The system of claim 3 , wherein the positioning system is configured to scan at least one additional fiber optic probes over the portion of the channel at a speed that is greater than the speed at which the sample is expected to flow through the channel.
6. The system of claim 1 , wherein a first fiber optic probe has a first field of view and a second fiber optic probe has a second field of view, and wherein a first portion of the channel is within the first field of view but not the second field of view and a second portion of the channel is within the second field of view but not the first field of view.
7. The system of claim 1 , wherein at least one of the probe heads comprises a ball lens or a gradient index lens.
8. The system of claim 1 , wherein the excitation source is optically connected to at least one of the optical fibers such that when the excitation source emits excitation light, the excitation light enters the optical fiber and then exits the optical fiber through the distal end of the optical fiber.
9. The system of claim 1 , wherein:
the microfluidic device further comprises a second channel for receiving a second sample of solution containing real-time PCR reagents, and
the imaging system further comprises at least one additional fiber optic probe comprising (i) an optical fiber having a distal end and a proximal end and (ii) a probe head connected to the distal end of the optical fiber and positioned between the distal end of the optical fiber and the second channel, and a second detector configured to detect emissions exiting the proximal end of the optical fiber of the second fiber optic probe.