Workflow timing between modules
The invention relates to a method of isolating and analyzing an analyte using an analytical apparatus which comprises modules of different types, wherein any one module of one type has a specific, pre-defined timing for carrying out its workflow.
1. A method of isolating and analyzing an analyte in an automated analyzer, comprising the steps of:
providing a liquid sample comprising said analyte to a processing vessel in a module of a first type;
transferring said liquid sample comprising said analyte to a module of a second type;
isolating and purifying said analyte in said processing vessel in said module of a second type;
transferring said purified analyte to a module of a third type comprising a temperature controlled incubator, wherein said module of a third type is an amplification module; and
analyzing said analyte in said temperature controlled incubator of said module of a third type by reacting said analyte with reagents necessary to obtain a detectable signal;
wherein the timing for transfer and processing within each module of one type is predefined, said timing for any one type of module is independent of the timing of any other type of module such that said modules of a first, second, and third type work autonomously, wherein isolation and purification of said analyte on the module of a second type is only started if, at the end of the isolation and purifying process, the amplification module is available, and wherein said timing in each module of one type is identical for any analyte which is isolated and analyzed; and
wherein the analyzer comprises at least two amplification modules.
2. The method of claim 1 , comprising a first analyte isolated and analyzed in said automated analyzer, and a second analyte isolated and analyzed in said automated analyzer, wherein said first and second analyte are isolated and analyzed in parallel, wherein said first analyte is analyzed in one of said amplification modules, and said second analyte is analyzed in a second one of said amplification modules, and wherein the time for isolating and analyzing said first and second analyte are identical.
3. The method of claim 1 , wherein said analyte is a nucleic acid analyte.
4. The method of claim 1 , wherein said automated analyzer comprises at least two modules of a second type.
5. The method of claim 1 , wherein said analyzer comprises at least four amplification modules.
6. The method of claim 1 , wherein at least 96 samples comprising at least two analytes are isolated and purified in parallel.
7. The method of claim 1 , wherein at least 192 samples comprising at least two analytes are isolated and purified in parallel in two separate modules of a second type, and are analyzed in four separate amplification modules, wherein the time for processing within any one of the modules of a second type is identical, and the time for processing within any one of the amplification modules is identical.
8. The method of claim 1 , wherein said transferring is automated.
9. The method of claim 1 , wherein said apparatus comprises a linear transfer module.
10. The method of claim 1 , wherein said apparatus comprises a rotational transfer module.
11. The method of claim 1 , wherein the time for isolating and purifying and analyzing any one analyte is identical to the time for isolating and purifying and analyzing any other analyte.