Repellent compositions and genetic approaches for controlling huanglongbing
The invention provides a method for controlling Huanglongbing (HLB) disease of citrus plants through expressing genes encoding synthases for sesquiterpenes such as β-caryophyllene, and α-copaene, and combinations thereof, in citrus plants. Methods of controlling HLB comprising applying at least one purified sesquiterpene, which repels Diaphorina citri and/or Tryoza erytrae psyllid insects, so as to control the HLB disease of citrus plants, are also disclosed.
1. A method for controlling Huanglongbing (HLB) in citrus plants comprising genetically modifying the citrus plants so that they express at least one isolated gene encoding a polypeptide having β-caryophyllene synthase to produce additional β-caryophyllene [sufficient] to repel Diaphorina citri and/or Tyroza erytrae psyllid insects so as to control HLB in the genetically modified plant; wherein the polypeptide is SEQ ID NO: 2.
2. The method of claim 1 , wherein the expression of the at least one isolated gene is driven by a constitutive promoter and a terminator region.
3. The method of claim 1 , wherein the expression of the at least one isolated gene is driven by a heterologous regulator region providing strong constitutive, tissue-specific or inducible expression.
4. The method of claim 3 , wherein the heterologous regulator region provides strong expression in the cytosol, chloroplasts or mitochondria.
5. The method of claim 1 , which further comprises expressing a gene encoding a farnesyl pyrophosphase synthase to enhance the accumulation of the β-caryophyllene produced by the polypeptide having β-caryophyllene synthase activity.
6. A method for controlling Huanglongbing (HLB) in citrus plants comprising genetically modifying the citrus plants so that they express at least one isolated gene encoding a polypeptide having β-caryophyllene synthase to produce additional β-caryophyllene in order to repel Diaphorina citri and/or Tryoza erytrae psyllid insects so as to control HLB in the genetically modified plant, wherein the isolated gene has SEQ ID NO: 1 and encodes a β-caryophyllene synthase (BCS) polypeptide that produces the additional β-caryophyllene to repel Huanglongbing (HLB).