HIV type and subtype detection
The present invention relates to the detection of HIV by amplification and PCR-based methods.
1. An amplicon obtainable by amplification from a HIV-containing sample with a primer of SEQ ID NO: 9 and a primer of SEQ ID NO: 24, wherein said amplicon is bound to at least one HIV nucleic acid, which comprises a fluorophore.
2. An amplicon obtainable by amplification from a HIV-containing sample with a primer of SEQ ID NO: 148 and a primer of SEQ ID NO: 170, wherein said amplicon is bound to at least one HIV nucleic acid, which comprises a fluorophore.
3. A set of oligonucleotides, comprising the oligonucleotides of SEQ ID NOs: 148 and 170, wherein said set further comprises at least one HIV nucleic acid, which comprises a fluorophore.
4. A set of oligonucleotides, comprising the oligonucleotides of SEQ ID NOs: 9 and 24, wherein said set further comprises at least one HIV nucleic acid, which comprises a fluorophore.
5. A set of oligonucleotides, which comprises at least two oligonucleotides, the respective sequences of which are structurally suitable as forward and reverse primers, respectively, for annealing to the L20587 HIV1-O reference isolate at such positions that they specifically amplify from said HIV reference isolate a sequence selected from the group consisting of SEQ ID NOs: 4 and 310-311 and the complementary sequences thereof, and which further comprises the oligonucleotides of SEQ ID NOs: 9 and 24, wherein said set further comprises at least one HIV nucleic acid which comprises a fluorophore.
6. A composition, which comprises:
at least one amplicon obtainable by amplification from a HIV-containing sample with a primer of SEQ ID NO.: 148 and a primer of SEQ ID NO: 170, wherein said amplicon is bound to at least one HIV nucleic acid, which comprises a fluorophore,
and which further comprises
at least two oligonucleotides, the respective sequences of which are structurally suitable as forward and reverse primers, respectively, for annealing to the K03455 HIV1-M reference isolate at such positions that they specifically amplify from said HIV reference isolate a sequence selected from the group consisting of:
SEQ ID NOs: 306-308 and
the complementary sequences thereof.
7. The amplicon of claim 1 , which is bound to a heat-activable polymerase, wherein said heat-activable polymerase is activable at 95° C.
8. The amplicon of claim 2 , which is bound to a heat-activable polymerase is activable at 95° C.
9. The set of claim 3 , which is bound to a activable polymerase, wherein said heat-activable polymerase is activable at 95° C.
10. The set of claim 4 , which is bound to a heat-activable polymerase, wherein said heat-activable polymerase is activable at 95° C.
11. The set of claim 5 , which is bound to a heat-activable polymerase, wherein said heat-activable polymerase is activable at 95° C.
12. The composition of claim 6 , which is bound to a heat-activable polymerase, wherein said heat-activable polymerase is activable at 95° C.
13. The amplicon of claim 1 , which is bound to a heat-activable polymerase, wherein said heat-activable polymerase is the Thermus aquaticus polymerase, or the Thermococcus litoralis polymerase, or the Pyrococcus furiosius polymerase.
14. The amplicon of claim 2 , which is bound to a heat-activable polymerase. wherein said heat-activable polymerase is the Thermus aquaticus polymerase, or the Thermococcus litoralis polymerase, or the Pyrococcus furiosius polymerase.
15. The set of claim 3 , which is bound to a heat-activable polymerase, wherein said heat-activable polymerase is the Thermus aquaticus polymerase, or the Thermococcus litoralis polymerase, or the Pyrococcus furiosius polymerase.
16. The set of claim 4 , which is bound to a heat-activable polymerase, wherein said heat-activable polymerase is the Thermus aquaticus polymerase, or the Thermococcus litoralis polymerase, or the Pyrococcus furiosius polymerase.
17. The set of claim 5 , which is hound to a heat-activable polymerase, wherein said heat-activable polymerase is the Thermus aquaticus polymerase, or the Thermococcus litoralis polymerase, or the Pyrococcus furiosius polymerase.
18. The composition of claim 6 , which is bound to a heat-activable polymerase, wherein said heat-activable polymerase is the Thermus aquaticus polymerase, or the Thermococcus litoralis polymerase, or the Pyrococcus furiosius polymerase.