Method of producing 3-hydroxypropionic acid from glycerol
The present invention relates to a novel method of producing 3-hydroxypropionic acid from glycerol, and more particularly to a method of producing 3-hydroxypropionic acid by culturing in a glycerol-containing medium a mutant microorganism obtained by amplifying an aldehyde dehydrogenase-encoding gene in a microorganism having the abilities to produce coenzyme B12 and produce 3-hydroxypropionic acid using glycerol as a carbon source. The present invention enables the fermentation of glycerol even under microaerobic or aerobic conditions without having to add coenzyme B12. Thus, the invention will be very suitable for the development of biological processes for producing large amounts of 3-hydroxypropionic acid.
1. A method for producing 3-hydroxypropionic acid by culturing Klebsiella pneumoniae AK-VOTHk (KCTC 11569BP) having the ability to produce coenzyme B12 and 3-hydroxypropionic acid using glycerol as a carbon source that has a deletion of the chromosomal glycerol dehydrogenase gene (DhaD), transcriptional activator gene (DhaR), 1,3-propanediol oxidoreductase gene (DhaT) and glycerol dehydratase reactivation factor II gene (DhaBA2) and is transformed with a plasmid comprising a 1,3-propanediol oxidoreductase-encoding gene, an aldehyde dehydrogenase-encoding gene, and a glycerol dehydratase gene (DhaB1), each under the control of an inducible promoter, the method comprising the steps of:
(a) culturing Klebsiella pneumoniae AK-VOTHk (KCTC 11569BP) in a glycerol-containing medium free of coenzyme B12, thereby producing 3-hydroxypropionic acid; and
(b) recovering the produced 3-hydroxypropionic acid.
2. A Klebsiella pneumoniae mutant, Klebsiella pneumoniae AK-VOTHk (KCTC 11569BP), having the ability to produce coenzyme B12 and 3-hydroxypropionic acid using glycerol as a carbon source that has a deletion of the chromosomal glycerol dehydrogenase gene (DhaD), transcriptional activator gene (DhaR), 1,3-propanediol oxidoreductase gene (DhaT) and glycerol dehydratase reactivation factor II gene (DhaBA2) and is transformed with a plasmid comprising a 1,3-propanediol oxidoreductase-encoding gene, an aldehyde dehydrogenase-encoding gene, and a glycerol dehydratase gene (DhaB1), each under the control of an inducible promoter.