Method of filtering platelets to remove antiplatelet and anticoagulant agents
The present invention relates to methods for removing antiplatelet agents and anticoagulants from a platelet preparation. In one embodiment, the method includes the step of flowing the platelet preparation through a filtering tube comprising a filtering membrane and separating the antiplatelet agents and anticoagulants from the platelet preparation by tangential flow filtration. In another embodiment, the method includes the step of passing the platelet preparation through porous material that specifically binds to the antiplatelet agents and anticoagulants.
1. A method for removing antiplatelet agents and anticoagulants from a platelet preparation, comprising:
flowing a platelet preparation comprising an antiplatelet agent, an anticoagulant and platelets through a filtering tube comprising a filtering membrane and separating the antiplatelet agent and anticoagulant from the platelet preparation by tangential flow filtration,
wherein the antiplatelet agent comprises a GPIIb/IIIa inhibitor and has a circulating half-life of inhibition of 4 hours or less and wherein the filter membrane has a pore size ranging from molecular cut off of 3000 daltons to 0.5 micron and wherein the anticoagulant comprises a short-to-ultrashort acting factor Xa inhibitor.
2. The method of claim 1 , wherein the filtering tube has an inner diameter of at least 0.5 mm.
3. The method of claim 1 , wherein the platelet preparation flows through the filtering tube at a flow rate of 150 to 400 ml/min.
4. The method of claim 1 , wherein an extraction liquid is circulated outside the filtering tube in a counter current manner.
5. The method of claim 4 , wherein the extraction fluid comprises 0.9% w/v sodium chloride.
6. The method of claim 1 , wherein the filtering membrane is made of a material selected from the group consisting of regenerated cellulose, cellulose acetate, polyamide, polyurethane, polypropylene, polysulfone, polyethersulfone, polycarbonate, nylon, polyimide and combinations thereof.
7. The method of claim 1 , wherein the filtering membrane is made of polysulfone or polyethylsulfone.
8. A method for removing antiplatelet agents and anticoagulants from a platelet preparation, comprising: flowing a platelet preparation comprising an antiplatelet agent, an anticoagulant and platelets along the surface of a filtering membrane and separating the antiplatelet agent and anticoagulant from the platelet preparation by tangential flow filtration, wherein the antiplatelet agent comprises a GPIIb/IIIa inhibitor and has a circulating half-life of inhibition of 4 hours or less and wherein the filter membrane has a pore size ranging from molecular cut off of 3000 daltons to 0.5 micron and wherein the anticoagulant comprises a short-to-ultrashort acting factor Xa inhibitor.
9. The method of claim 8 , wherein the filtering membrane is made of a material selected from the group consisting of regenerated cellulose, cellulose acetate, polyamide, polyurethane, polypropylene, polysulfone, polyethersulfone, polycarbonate, nylon, polyimide and combinations thereof.
10. The method of claim 8 , wherein the filtering membrane is made of polysulfone or polyethylsulfone.