Nucleic acid of recombination expression vector encoding soluble forms of CD83, host cells transformed/transfected therewith and pharmaceutical compositions containing same
The present invention provides methods of using soluble forms of CD83 and nucleic acids encoding them for the treatment or prevention of diseases caused by the dysfunction or undesired function of a cellular immune response involving T cells. The invention moreover provides soluble CD83 proteins, specifically suited for said purpose, nucleic acids and vectors encoding said CD83 proteins, cells transformed/transfected with the nucleic acids or vectors, methods for producing said CD83 proteins, pharmaceutical compositions containing said CD83 proteins, nucleic acids or vectors, and antibodies against said soluble CD83 proteins and assay methods and kits comprising said antibodies.
1. A nucleic acid or recombinant expression vector encoding a soluble CD83 protein, said nucleic acid being a cDNA sequence, and said soluble CD83 protein being selected from the group consisting of:
a) a protein consisting of amino acid residues 20-145 of SEQ ID NO: 2;
b) a protein consisting of amino acid residues 1-130 of SEQ ID NO: 8;
c) the protein of SEQ ID NO: 8; and
d) the protein which consists of the amino acid sequence of any one of said proteins a) through c) but has one conservative amino acid substitution.
2. A prokaryotic or eukaryotic host cell transformed/transfected with a nucleic acid or a vector of claim 1 .
3. A method for producing a soluble CD83 protein, which comprises culturing a transferred/transfected prokaryotic or eukaryotic host cell according to claim 2 .
4. The nucleic acid or recombinant expression vector of claim 1 , which encodes a CD83 protein consisting of amino acid residues −9 to 130 of SEQ ID NO: 8.
5. The nucleic acid or recombinant expression vector of claim 1 , which encodes a CD83 protein consisting of the protein of SEQ ID NO: 8.
6. The nucleic acid or recombinant expression vector of claim 1 , which encodes a CD83 protein consisting of the protein which consists of the amino acid sequence of any one of said proteins a) through c) but has one conservative amino acid substitution.
7. A nucleic acid or recombinant expression vector comprising a start codon operatively linked to a sequence encoding a soluble CD83 protein, said nucleic acid being a cDNA sequence, and said soluble CD83 protein being selected from the group consisting of:
a) a protein consisting of amino acid residues 20-145 of SEQ ID NO: 2;
b) a protein consisting of amino acid residues 1-130 of SEQ ID NO: 8;
c) the protein of SEQ ID NO: 8;
d) the protein which consists of the amino acid sequence of any one of said proteins a) through c) but has one conservative amino acid substitution.