Primer and probe sequences for detecting
The present invention relates to primers and probes that can be used in various assays to detect a new strain of Chlamydia trachomatis . The invention further provides for the simultaneous detection of other diseases, especially Neisseria gonorrhoeae.
1. A set of polynucleotides comprising a first, a second, and a third isolated polynucleotide, wherein
the first polynucleotide consists of SEQ ID NO: 1, or the complement thereof, wherein the complement is identical in length to SEQ ID NO: 1;
the second polynucleotide consists of SEQ ID NO: 2, or the complement thereof, wherein the complement is identical in length to SEQ ID NO: 2; and
the third polynucleotide consists of SEQ ID NO:3, or the complement thereof, wherein the complement is identical in length to SEQ ID NO:3, and wherein the third polynucleotide comprises a label and a quencher.
2. The polynucleotide set of claim 1 , further comprising a fourth, a fifth and a sixth isolated polynucleotide, wherein
the fourth polynucleotide consists of SEQ ID NO: 28, or the complement thereof, wherein the complement is identical in length to SEQ ID NO: 28;
the fifth polynucleotide consists of SEQ ID NO: 31, or the complement thereof, wherein the complement is identical in length to SEQ ID NO: 31; and
the sixth polynucleotide consists of SEQ ID NO: 33, or the complement thereof, wherein the complement is identical in length to SEQ ID NO: 33.
3. The polynucleotide set of claim 2 , wherein the sixth polynucleotide comprises a label and a quencher.
4. A kit, comprising the polynucleotide set of claim 1 and amplification reagents.
5. A method of amplifying a Chlamydia trachomatis nucleic acid sequence in a sample, comprising
(a) forming a reaction mixture comprising amplification reagents, a sample suspected of containing a Chlamydia trachomatis nucleic acid sequence and the set of polynucleotides of claim 1 ; and
(b) subjecting the reaction mixture to conditions to promote the amplification reagents to amplify at least one copy of a nucleic acid sequence complementary to the third polynucleotide.
6. The method of claim 5 , wherein the reaction mixture further comprises a control target polynucleotide and a control polynucleotide probe.
7. A method of detecting Chlamydia trachomatis in a sample, comprising
(a) forming a reaction mixture comprising amplification reagents, a sample suspected of containing a Chlamydia trachomatis nucleic acid sequence and the set of polynucleotides of claim 1 ;
(b) subjecting the reaction mixture to conditions to promote the amplification reagents to amplify at least one copy of a nucleic acid sequence complementary to the third polynucleotide;
(c) subjecting the reaction mixture to conditions to promote specific hybridization of the third polynucleotide to a target sequence; and
(d) detecting third polynucleotide:target sequence hybrids.
8. The method of claim 7 , wherein the reaction mixture further comprises a control target polynucleotide and a control polynucleotide probe.