Accurate and rapid micromixer for integrated microfluidic devices
The invention may provide a microfluidic mixer having a droplet generator and a droplet mixer in selective fluid connection with the droplet generator. The droplet generator comprises first and second fluid chambers that are structured to be filled with respective first and second fluids that can each be held in isolation for a selectable period of time. The first and second fluid chambers are further structured to be reconfigured into a single combined chamber to allow the first and second fluids in the first and second fluid chambers to come into fluid contact with each other in the combined chamber for a selectable period of time prior to being brought into the droplet mixer.
1. A microfluidic mixer, comprising:
a droplet generator comprising a main channel; and
a droplet mixer in selective fluid connection with said droplet generator,
wherein a portion of said main channel is structured to be reconfigured into first and second fluid chambers with one of a first and second valve at each opposing end of said first and second fluid chambers and a third valve separating said first and second fluid chambers,
wherein said first fluid chamber has a first fluid input channel that is separable from said first fluid chamber by a first fluid input valve situated at the periphery of said first fluid chamber,
wherein said second fluid chamber has a second fluid input channel that is separable from said second fluid chamber by a second fluid input valve situated at the periphery of said second fluid chamber,
wherein said first and second fluid chambers are structured to be at least partially filled with respective first and second fluids that can each be held in isolation for a selectable period of time,
wherein said first and second fluid chambers are further structured to be reconfigured into a single combined chamber to allow said first and second fluids in said first and second fluid chambers to come into fluid contact with each other in said combined chamber for a selectable period of time prior to being brought into said droplet mixer, and
wherein said main channel is configured to contain said first and second fluids that have come into fluid contact with each other outside said first and second fluid chambers upon opening of said first and second valves.
2. The microfluidic mixer according to claim 1 , wherein at least one of said first fluid chamber and said second fluid chamber has a volume that is selectable from a plurality of volumes.
3. The microfluidic mixer according to claim 2 , wherein said droplet generator comprises a plurality of valves that can be selectively opened and closed to provide said volume of said at least one of said first and second fluid chambers that is selectable from a plurality of volumes.
4. The microfluidic mixer according to claim 1 , wherein said first fluid input channel is structured to allow delivery of said first fluid to said first fluid chamber and said second fluid input channel is structured to allow delivery of said second fluid to said second fluid chamber.
5. The microfluidic mixer according to claim 4 , wherein said first and second fluid input channels are structured to allow delivery of first and second fluids that are different from each other.
6. The microfluidic mixer according to claim 1 , wherein said droplet mixer comprises a microchannel in fluid connection with said main channel of said droplet generator to receive droplets from said droplet generator while in operation.
7. The microfluidic mixer according to claim 6 , wherein said microchannel has a serpentine shaped path.
8. The microfluidic mixer according to claim 1 , further comprising a degasser in fluid connection with said droplet mixer, said degasser being structured to remove gas at least one of from or between droplets generated by said droplet generator.
9. The microfluidic mixer according to claim 8 , wherein said degasser comprises a droplet channel and an evacuation channel, said droplet and evacuation channels having a region of close approach with a gas-permeable membrane therebetween such that when said evacuation channel is under at least a partial vacuum, gas can be exchanged from said droplet channel to said evacuation channel while in operation.
10. The microfluidic mixer according to claim 1 , wherein a second portion of said main channel is structured to be reconfigured into a third fluid chamber with a fourth valve at one end and one of said first and second valves at an end opposing said fourth valve,
wherein said third fluid chamber has a third fluid input channel that is separable from said third fluid chamber by a third fluid input valve situated at the periphery of said third fluid chamber,
wherein said third fluid chamber is structured to be at least partially filled with a third fluid such that said first, second and third fluids can each be held in isolation for a selectable period of time, and
wherein said first, second and third fluid chambers are further structured to be reconfigured into a single combined chamber to allow said first, second and third fluids in said first, second and third fluid chambers to come into fluid contact with each other in said combined chamber for a selectable period of time prior to being brought into said droplet mixer.
11. The microfluidic mixer according to claim 1 , wherein said microfluidic mixer is adapted to be fluidly connected to at least one other microfluidic device.
12. The microfluidic mixer according to claim 1 , wherein said first fluid chamber has a first evacuation channel that is separable from said first fluid chamber by a first evacuation valve situated at the periphery of said first fluid chamber, and wherein said second fluid chamber has a second evacuation channel that is separable from said second fluid chamber by a second evacuation valve situated at the periphery of said second fluid chamber.