Methods of identifying glycopeptides recognized by disease-associated auto-antibodies
Methods for identifying glycopeptides and more particularly glycopeptide epitopes that are specifically recognized by disease-associated auto-antibodies are provided. In some aspects the auto-antibodies are cancer-associated or autoimmune disease associated. In other aspects, methods of diagnosing a patient with cancer or an autoimmune disease, or for eliciting an immune response in a mammalian host directed to the glycopeptides of the invention are provided.
1. A panel of glycopeptides comprising at least a plurality of isolated glycopeptides, each isolated glycopeptide comprising a glycopeptide epitope, said epitope having been previously determined (i) to be selectively recognized by a subset of antibodies in sera from cancer patients, which subset recognizes neither (a) the corresponding naked peptides of said panel when not glycosylated; nor (b) the corresponding glycan when not bound to said peptide; and (ii) not to be recognized by antibodies in control sera, said plurality comprising at least 8 isolated glycopeptides, wherein said plurality comprises isolated glycopeptides comprising amino acid sequences of SEQ ID NOs: 86, 109, 116, 132, 134, 135, 145, and 146, wherein said glycopeptides are immobilized on said panel.
2. The panel of glycopeptides of claim 1 , wherein the plurality of isolated glycopeptides comprises SHHSDESDELVTDFPTDLPA (SEQ ID NO: 15); TPTPKEKPEAGTYSVNNGND (SEQ ID NO: 36); SESFPHPGFNMSLLENHTRQ (SEQ ID NO: 49); LAKMYYSAVEPTKDIFTGLI (SEQ ID NO: 86); TDCGGPKDHPLTCDDPRFQA (SEQ ID NO: 109); PGTSTTPSQPNSAGVQDTEM (SEQ ID NO: 116); TKTDASSTHHSTVPPLTSSN (SEQ ID NO: 132); HDVETQFNQYKTEAASRYNL (SEQ ID NO: 134); ASRYNLTISDVSVSDVPFPF (SEQ ID NO: 135); VPVTRPALGSTTPPAHDVTS (SEQ ID NO: 145); and SLASQATDTFSTVPPTPPSI (SEQ ID NO: 146).
3. The panel of glycopeptides of claim 1 , wherein the plurality comprises at least 8 and up to 30 isolated glycopeptides, wherein the plurality comprises isolated glycopeptides having amino acid sequences SEQ ID NOs: 86, 109, 116, 132, 134, 135, 145, and 146.