Methods and compositions for regulation of transgene expression
Nucleases and methods of using these nucleases for expressing a transgene from a safe harbor locus in a secretory tissue, and clones and animals derived therefrom.
1. A non-naturally occurring fusion protein comprising
(i) a Transcription Activator Like Effector (TALE) DNA binding domain that binds to an endogenous albumin gene, wherein the TALE DNA binding protein comprises a plurality of TALE repeat units, each TALE repeat unit comprising an amino acid Repeat Variable Diresidue (RVD) that binds to a nucleotide in a target sequence in an endogenous albumin gene, wherein the TALE DNA binding domain comprises a +17 or +63 C-terminal truncation and
(ii) a cleavage domain,
wherein the non-naturally occurring fusion protein cleaves the endogenous albumin gene.
2. A kit comprising the non-naturally occurring fusion protein according to claim 1 .