Collagen materials and methods for obtaining same
The invention relates to novel collagen materials and, specifically, collagen membranes, tubes and threads. Said materials combine enhanced properties of resilience and strength. The invention also relates to a method for preparing collagen materials using acid fibrous collagen comprising coagulation and, optionally, cross-linking of the collagen in the presence of ammonia gas.
1. A method for preparing a collagen material comprising the following steps, performed in the following order:
a) preparing an aqueous solution of native or denatured collagen, wherein the aqueous collagen solution is at an acidic pH,
b) adding an oxidized polysaccharide comprising an aldehyde group as a cross-linking agent that is non-reactive at acidic pH to the aqueous collagen solution of step a),
c) molding or casting the aqueous collagen solution of step b),
d) coagulating and cross-linking the molded or casted aqueous collagen solution of step c) by treating the molded or casted aqueous collagen solution with ammonia gas, wherein cross-linking occurs at least partially concomitantly with the coagulation of the collagen, and
e) removing excess ammonia and obtaining said collagen material by drying.
2. The method of claim 1 , wherein the aqueous solution of step a) comprises 0.05% to 3% by weight of collagen in acid form.
3. The method of claim 1 , wherein the aqueous solution of step a) is prepared with acidic fibrous collagen selected from the group consisting of collagens of pig tendons, calf tendons, lamb tendons and foal tendons.
4. The method for preparing a collagen material according to of claim 1 , wherein in step d), coagulation and cross-linking of the collagen solution are carried out by treating with ammonia gas for at least 24 hours.
5. The method of claim 1 , wherein the cross linking agent oxidized polysaccharide comprising an aldehyde group is selected from the group consisting of glycogen and aldehyde amylopectins.
6. The method of claim 5 , wherein the oxidized polysaccharide comprising an aldehyde group is oxidized glycogen.
7. The method of claim 1 , wherein the oxidized polysaccharide comprising an aldehyde group is added in a ratio ranging from 0.05 to 5 of the aldehyde —CHO of the oxidized polysaccharide to the —NH 2 of the collagen.
8. The method for of claim 1 , wherein the collagen material is a membrane, wherein in step c) the aqueous collagen solution is deposited on a flat mold and wherein in step e) excess ammonia is removed and a collagen membrane is obtained by drying.
9. The method of claim 1 , wherein molding or casting the aqueous collagen solution comprises molding the aqueous solution into a collagen membrane, a collagen film, a collagen thread, a collagen tube, a collagen sponge or a collagen gel.
10. A method for preparing a fabric-reinforced collagen membrane comprising the following steps, performed in the following order:
a) preparing an aqueous solution of native or denatured collagen, wherein the aqueous collagen solution is at an acidic pH,
b) adding an oxidized polysaccharide comprising an aldehyde group as a cross-linking agent that is non-reactive at acidic pH to the aqueous collagen solution of step a),
c) molding or casting the aqueous collagen solution of step b) and depositing a textile on the molded or casted aqueous collagen solution,
d) coagulating and cross-linking the molded or casted aqueous collagen solution, which contains the cross-linking agent of step c), by treating the molded or casted aqueous collagen solution with ammonia gas, wherein cross-linking occurs at least partially concomitantly with the coagulation of the collagen, and
e) removing excess ammonia and obtaining said fabric-reinforced collagen membrane by drying.