Method for preparing immobilized angiogenin
Stabilized angiogenin compositions and methods of preparing a stabilized angiogenin compositions by covalent immobilization on a naturally occurring substrate, such as a polysaccharide substrate, are disclosed. In particular, the polysaccharide substrate includes galactose-rich polysaccharide.
1. A method of preparing immobilized angiogenin comprising mixing angiogenin with a polysaccharide substrate in a liquid medium, wherein the polysaccharide comprises (1) galactose, (2) anhydrogalactose, (3) 2-O-methyl-galactose, (4) 4-O-methyl-galactose or combinations of (1) to (4), (5) carrageenans, or combinations of (1) to (5), wherein the angiogenin covalently attached to the polysaccharide substrate.
2. The method of claim 1 , wherein the immobilization occurs by attachment of the N-terminus of angiogenin to the polysaccharide substrate.
3. The method of claim 1 , wherein the polysaccharide substrate is extracted from water-soluble fraction of agar and comprises galactose derivatives comprising anhydrogalactose, 2-O-methyl galactose, 4-O-methyl galactose or combinations thereof.
4. The method of claim 1 , wherein the liquid medium is water.
5. The method of claim 1 , wherein the polysaccharide comprises carrageenans.
6. The method of claim 5 , wherein the immobilization occurs by attachment of the N-terminus of angiogenin to the polysaccharide substrate.
7. The method of claim 5 , wherein the liquid medium is water.