Biologic fluid analysis cartridge with deflecting top panel
A cartridge for analyzing a biologic fluid sample is provided that includes a base plate, a sample inlet port, a first chamber wall, a second chamber wall, and an optically transparent cover panel disposed in contact with the first and second chamber walls. The base plate has a body with a chamber surface, a body passage, and a chamber entry passage. The body passage is in fluid communication with the chamber entry passage, and the chamber entry passage extends through to the chamber surface. The sample inlet port has an inlet passage in fluid communication with the body passage. The first and second chamber walls each have a height extending outwardly from the chamber surface, and the two walls are spaced apart from one another. The cover panel is sufficiently flexible to deflect and contact a central region of the chamber surface.
1. A cartridge for analyzing a biologic fluid sample, comprising:
a base plate having a body with a chamber surface, a body passage, and a chamber entry passage, wherein the body passage is in fluid communication with the chamber entry passage, and the chamber entry passage extends through to the chamber surface;
a sample inlet port having an inlet passage in fluid communication with the body passage;
a first chamber wall having a height extending outwardly from the chamber surface;
a second chamber wall having a height extending outwardly from the chamber surface, spaced apart from the first chamber wall; and
a cover panel disposed in contact with the first and second chamber walls, wherein the cover panel, first and second chamber walls, and the chamber surface define an analysis chamber;
wherein the cover panel is optically transparent, and the cover panel includes a material which enables the cover panel to be sufficiently flexible to deflect and contact a central region of the chamber surface when subjected to capillary forces from the sample quiescently residing between the cover panel and the base plate chamber surface, and thereby separate the analysis chamber into a first sub-chamber disposed on a first side of the contact between the cover panel and the central region, and into a second sub-chamber independent from the first sub-chamber on a second side of the contact between the cover panel and the central region, opposite the first side.
2. The cartridge of claim 1 , wherein a first reagent is disposed in the first sub-chamber and a second reagent is disposed in the second sub-chamber, wherein the first reagent is different from the second reagent.
3. The cartridge of claim 1 , wherein the height of the first chamber wall is greater than the height of the second chamber wall.
4. The cartridge of claim 1 , wherein the base plate further includes a manifold, a plurality of body passages, and a plurality of analysis chambers, wherein the inlet passage is in fluid communication with the manifold, and each body passage is in fluid communication with the manifold and one of the analysis chambers.
5. The cartridge of claim 1 , further comprising a calibration reference.
6. The cartridge of claim 1 , further comprising a plurality of separators of uniform height disposed in the central region.
7. The cartridge of claim 1 , wherein the material is a film material.
8. The cartridge of claim 7 , wherein the material is a polyester film material.