Process for producing fermentation products and fermentation medium compositions therefor
View Patent ↗Fermentation products (e.g., bioproducts such as hydrocarbons and other organic compounds) are produced from biomass or gases through digestion and fermentation under conditions that thermodynamically favor production of the fermentation products.
1. A method for converting a substrate to a C 2 -C 8 alkane, comprising the steps:
a) adding the substrate to a fermentation vessel;
b) supplying a source of hydrogen gas to the fermentation vessel to increase the partial pressure of hydrogengas in the fermentation vessel to at least 0.001 atmospheres;
c) fermenting the substrate in the fermentation vessel with a microbial culture that converts the substrate to the C 2 -C 8 alkane; and
d) removing the alkane.
2. The method of claim 1 , wherein the C 2 -C 8 alkane is selected from a group consisting of ethane, propane, n-butane, and iso-butane.
3. The method of claim 1 , wherein the C 2 -C 8 alkane is selected from a group consisting of pentane, hexane, heptane, and octane.
4. The method of claim 1 , wherein gases in the fermentation vessel are at a total pressure of about 1 to about 4 atmospheres.
5. A method for converting a substrate to a C 2 -C 8 alkane, comprising the steps:
a) adding the substrate to a fermentation vessel;
b) supplying a source of hydrogen gas to the fermentation vessel, wherein ratio of H 2 :CO 2 by volume is about or at least 3:1, or ratio of H 2 :CO by volume is about or at least 2:1;
c) fermenting the substrate in the fermentation vessel with a microbial culture that converts the substrate to the C 2 -C 8 alkane; and
d) removing the alkane.
6. The method of claim 1 , wherein the substrate comprises gases H 2 and CO 2 or CO, which are converted to the alkane and H 2 O by the microbial culture.
7. The method of claim 1 , wherein the substrate comprises a carboxylic acid, and the microbial culture hydrogenates the carboxylic acid to produce the alkane and H 2 O.
8. The method of claim 1 , wherein the substrate comprises biomass.
9. The method of claim 8 , wherein the biomass comprises cellulose.
10. The method of claim 1 , wherein temperature of broth in the vessel is greater than 20° C. and less than 60° C.
11. The method of claim 6 , further comprising mathematically determining the reaction conditions needed to produce the alkane at a desired concentration from the gases by calculating ΔG values for a plurality of alternative reaction conditions for converting the gases to the alkane and ΔG values for converting the gases to acetate, and selecting a set of reaction conditions that make it thermodynamically favorable to produce the alkane at a desired concentration over producing the acetate; and adding the gases or the acetate or removing the alkane to establish the reaction conditions needed to produce the alkane at the desired concentration.
12. The method of claim 11 , wherein the alternative reaction conditions include different partial pressures of gases comprising H 2 , CO 2 , or CO.
13. A method for isolating microorganisms that convert a substrate to a C 2 -C 8 alkane comprising: collecting a sample from a source containing microorganisms that produce the alkane; growing the microorganisms in a culture in a fermentation vessel;
supplying a source of hydrogen gas to the fermentation vessel to increase partial pressure of hydrogen gas in the fermentation vessel to at least 0.001 atmospheres; and isolating one or more microorganisms from the culture.
14. A process to ferment a substrate to a C 2 -C 8 alkane comprising;
adding the substrate and an isolated microorganism to a fermentation vessel;
infusing a source of hydrogen gas into the fermentation vessel to increase the partial pressure of hydrogengas in the fermentation vessel to at least 0.001atmospheres; wherein the isolated microorganism produces the C 2 -C 8 alkane; and removing the C 2 -C 8 alkane.
15. The process of claim 14 , wherein the substrate comprises CO 2 , or CO.
16. The process of claim 14 , wherein the substrate comprises a carboxylic acid.
17. The process of claim 14 , wherein the isolated microorganism is anaerobic.
18. The process of claim 14 , wherein the isolated microorganism contains 16S rRNA that is at least 97% homologous with that of a member of a taxonomic genus from among Actinomyces, Enterococcus, Eschericia, Clostridium, Proteus , or Tissierella.
19. The process of claim 1 , wherein partial pressure of hydrogen gas in the fermentation vessel is at least 0.05 atmospheres.