Methods for enzymatic treatment of wool
Disclosed are methods for enzymatic treatment of wool using bacterial protease, cellulase, and xylanase enzymes.
1. A method for enzymatic treatment of wool fibres, the method comprising contacting the wool fibres with a composition comprising at least one protease, at least one cellulase, at least one xylanase, and a plurality of calcium hydroxyapatite nanoparticles.
2. The method of claim 1 , wherein the protease, the cellulase, and the xylanase are bacterial enzymes.
3. The method of claim 1 , wherein the contacting step is performed at a temperature of about 25° C. to about 70° C.
4. The method of claim 1 , wherein the contacting step is performed at pH of about 2.0 to about 10.0.
5. The method of claim 1 , wherein the contacting step is performed for a period of about 3 hours to about 15 hours.
6. A composition for enzymatic treatment of wool fibres, the composition comprising at least one protease, at least one cellulase, at least one xylanase, and a plurality of calcium hydroxyapatite nanoparticles.
7. The composition of claim 6 , wherein the protease, the cellulase, and the xylanase are bacterial enzymes.
8. The composition of claim 6 , wherein the composition has a pH of about 2.0 to about 10.0.
9. A kit for enzymatic treatment of wool fibres, the kit comprising:
at least one protease;
at least one cellulase;
at least one xylanase;
a plurality of calcium hydroxyapatite nanoparticles; and
instructions for use.
10. The kit of claim 9 , wherein the protease, cellulase, and xylanase are bacterial enzymes.