Moisture-activated self-heating analysis device
Provided are disposable, moisture-activated, self-heating cartridges useful for, e.g., isothermal nucleic acid amplification, incubation, and thermal actuation, and visual fluorescent detection of the amplification products. These devices may be self-contained and do not require any special instruments to operate.
1. A device, comprising:
a heating material that undergoes an exothermic reaction upon contact with moisture;
a thermal storage medium in thermal communication with the moisture-activated heating source, the thermal storage medium comprising a phase-changing material; and
a conduit placing the heating material in fluid communication with a reservoir adapted to contain a fluid, the conduit comprising a material capable of transporting liquid by capillary action,
wherein the heating material is in thermal communication with an enzymatic amplification reaction chamber, a thermally-actuated fluidic element, or any combination thereof.
2. The device of claim 1 , wherein the conduit comprises a fibrous material.
3. The device of claim 2 , wherein the fibrous material comprises filter paper, cellulose, polytetafluoroethylene (PTFE), nitrocellulose, an acrylic copolymer, polypropylene, polyethylene, polyvinylidene fluoride (PVDF), or any combination thereof.
4. The device of claim 1 , wherein the conduit comprises a material comprising two or more different fiber sizes, two or more different pore sizes, or both.
5. The device of claim 1 , wherein the enzymatic amplification chamber is adapted for isothermal amplification.
6. A device, comprising:
a heating material that undergoes an exothermic reaction upon contact with moisture;
a thermal storage medium in thermal communication with the moisture-activated heating source, the thermal storage medium comprising a phase-changing material; and
a conduit placing the heating material in fluid communication with a reservoir adapted to contain a fluid, the conduit comprising a material capable of transporting liquid by capillary action,
wherein the heating material is in thermal communication with an incubation and culture growth reaction chamber, a thermally-actuated fluidic element, or any combination thereof.
7. The device of claim 1 , wherein the thermally-actuated fluidic element comprises a valve, a piston, a membrane, a cantilever, thermally-releasable reagents, or any combination thereof.
8. The device of claim 1 , wherein the thermal storage medium comprises a wax, a thermoplastic, a salt hydrate, a fatty acid, a fatty acid ester, or any combination thereof.
9. The device of claim 1 , wherein the heating material comprises magnesium-iron alloy, calcium oxide, sodium acetate, potassium permanganate, or any combination thereof.
10. The device of claim 6 , wherein the thermally-actuated fluidic element comprises a valve, a piston, a membrane, a cantilever, thermally-releasable reagents, or any combination thereof.