Instruments for biological sample preparation devices
Methods and devices for biological sample preparation and analysis are disclosed. A device may have a linear or circular arrangement of containers, with a connecting structure such as a bar or disk. Fluidics channels between containers allow the performance of different techniques for sample preparation, such as lysing, washing and elution. Different functional elements, such as grinders or mixers, may be attached to the containers.
1. An instrument comprising:
a recess;
a sample preparation device;
a supporting element, configured to attach to the sample preparation device to secure the sample preparation device in the recess inside the instrument;
at least one motorized actuator, configured to actuate plungers of the sample preparation device that are arranged in a circular pattern, wherein the at least one motorized actuator comprises
a motor comprising a rotating rod having a rotating axis,
a fixed plate attached to the rotating axis, and
a linear actuator having a first end and a second end, wherein the first end is attached to the fixed plate, and the second end is attached to an actuator rod, the actuator rod having an axis parallel to the rotating axis of the motor, and wherein the actuator rod is configured to operate the plungers;
a controlling interface, configured to accept input from a user and operate the at least one motorized actuator based on the input from the user; and
a housing structure, containing the recess, the supporting element, the at least one motorized actuator, and the controlling interface.
2. The instrument of claim 1 , wherein the sample preparation device has a top structure with a first plurality of containers and a bottom structure with a second plurality of containers, and the sample preparation device comprises a plunger for each of the first and second plurality of containers.
3. The instrument of claim 1 , wherein the supporting element further comprises a heater plate.
4. The instrument of claim 1 , wherein the controlling interface comprises a microcontroller, at least one memory chip, and a motorized actuator interface.
5. The instrument of claim 3 , wherein the controlling interface further comprises a heater driver controlling the heater plate and is further configured to operate the heater driver based on the input from the user.
6. The instrument of claim 4 , wherein the controlling interface further comprises a USB chip, a Bluetooth chip, a Firewire chip, an Ethernet chip, or a Wireless chip.
7. The instrument of claim 5 , further comprising a temperature sensor.
8. The instrument of claim 1 , further comprising motion control sensors, wherein the controlling interface is configured to interface with the motion control sensors to control the at least one motorized actuator.
9. The instrument of claim 1 , wherein the controlling interface can be operated through a smartphone, a tablet, a laptop or a desktop computer.
10. The instrument of claim 1 , further comprising a reaction chamber, a moving plate to engage the reaction chamber, a solenoid to operate the moving plate, an optical element and a waveguide.
11. The instrument of claim 10 , wherein the optical element comprises a light source, a lens and an optical excitation filter.
12. The instrument of claim 10 , wherein the reaction chamber includes a prism and the waveguide is configured to direct electromagnetic rays into the prism.