Pathogen detection biosensor
The invention described herein provides methods for the detection of target particles, such as pathogens, soluble antigens, nucleic acids, toxins, chemicals, plant pathogens, blood borne pathogens, bacteria, viruses and the like. Also described is an emittor cell comprising a receptor, wherein the receptor can be an antibody or an Fc receptor, and an emittor molecule for the detection of a target particle in a sample wherein the target particle to be detected is bound by one or more receptors on the emittor cell. Also provided are optoelectronic sensor devices for detecting a target particle in a sample, including in a plurality of samples.
1. A method for detecting an antigen in a sample comprising:
a) spraying emitter cells onto a sample, wherein the emitter cell comprises a receptor and an emitter molecule that emits a photon in response to binding of a target antigen in the sample to the receptor;
b) detecting photon emission, wherein photon emission is indicative of the antigen in the sample.
2. The method of claim 1 , wherein the emitter cells are sprayed onto the sample using an atomizer.
3. The method of claim 1 , wherein the emitter cells are sprayed onto a liquid sample.
4. The method of claim 1 , wherein the emitter cells are sprayed onto a solid surface comprising said sample.
5. The method of claim 1 , wherein the emitter cells are sprayed onto a solid surface comprising a dried sample.
6. The method of claim 4 , wherein the solid surface is a centrifuge tube.