IP Library Granted Patent US 9,309,530
Granted Patent B2
US 9,309,530 · App. 14/815,295 · Granted Apr 12, 2016

Gene capable of improving material productivity in seed and method for use thereof

Inventors: Satoshi Kondo (Miyoshi, JP); Chikara Ohto (Toyota, JP); Nobuhiko Muramoto (Ichinomiya, JP); Norihiro Mitsukawa (Miyoshi, JP); Masaru Takagi (Tsuchiura, JP); Kyoko Matsui (Ryugasaki, JP)
Assignee: TOYOTA JIDOSHA KABUSHIKI KAISHA
C12N15/8247A01H5/02C07K14/415C12N15/8216C12N15/8217
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Quick Facts
Patent No.
US 9,309,530
App. No.
14/815,295
Granted
Apr 12, 2016
Kind
B2
Abstract

An object of the present invention is to search for a gene having a novel function that can cause an increase or decrease in material productivity, and particularly, fat and oil content. In the present invention, a chimeric protein obtained by fusing a transcription factor consisting of a protein comprising an amino acid sequence shown in any of the even-numbered SEQ ID NOS: 1 to 158 and a functional peptide capable of converting an arbitrary transcription factor into a transcriptional repressor is expressed in a plant.

Claims (31)

1. A method for significantly improving oil productivity in an individual seed, as compared to a seed from a plant not comprising the chimeric protein, by causing expression of a chimeric protein obtained by fusing a transcription factor consisting of any one of the following proteins (a) to (b) and a functional peptide capable of converting an arbitrary transcription factor into a transcriptional repressor in a plant:

(a) a protein comprising the amino acid sequence of SEQ ID NO: 6; and

(b) a protein having transactivation activity and comprising an amino acid sequence that has a deletion, a substitution, an addition, or an insertion, of one to ten amino acids with respect to SEQ ID NO: 6.

2. The method according to claim 1 , wherein transactivation activity of the transcription factor is repressed.

3. The method according to claim 1 , wherein the chimeric protein has transcriptional repressor activity.

4. The method according to claim 1 , wherein the functional peptide has an amino acid sequence expressed by any one of the following formulae (1) to (8):

(1) X1-Leu-Asp-Leu-X2-Leu-X3 (SEQ ID NO: 520 with deletion of 0-10 residues from the N-terminus) (where X1 denotes a set of 0 to 10 amino acid residues, X2 denotes Asn or Glu, and X3 denotes a set of at least 6 amino acid residues);

(2) Y1-Phe-Asp-Leu-Asn-Y2-Y3 (SEQ ID NO: 521 with deletion of 0-10 residues from the N-terminus) (where Y1 denotes a set of 0 to 10 amino acid residues, Y2 denotes Phe or Ile, and Y3 denotes a set of at least 6 amino acid residues);

(3) Z1-Asp-Leu-Z2-Leu-Arg-Leu-Z3 (SEQ ID NO: 522 with deletion of 0-10 residues from the C-terminus and deletion of 0-2 residues from the N-terminus) (where Z1 denotes Leu, Asp-Leu, or Leu-Asp-Leu, Z2 denotes Glu, Gln, or Asp, and Z3 denotes a set of 0 to 10 amino acid residues);

(residues 4-9 of SEQ ID NO: 522)

(4)

Asp-Leu-Z4-Leu-Arg-Leu

(where Z4 denotes Glu, Gln, or Asp);

(SEQ ID NO: 523)

(5)

α1-Leu-β1-Leu-γ1-Leu;

(SEQ ID NO: 524)

(6)

α1-Leu-β1-Leu-γ2-Leu;

(SEQ ID NO: 525)

(7)

α1-Leu-β2-Leu-Arg-Leu;

and

(SEQ ID NO: 526)

(8)

α2-Leu-β1-Leu-Arg-Leu

(where α1 denotes Asp, Asn, Glu, Gln, Thr, or Ser, α2 denotes Asn, Glu, Gln, Thr, or Ser, β1 denotes Asp, Gln, Asn, Arg, Glu, Thr, Ser, or His, β2 denotes Asn, Arg, Thr, Ser, or His, γ1 denotes Arg, Gln, Asn, Thr, Ser, His, Lys, or Asp, and γ2 denotes Gln, Asn, Thr, Ser, His, Lys, or Asp in formulae (5) to (8)).

5. The method according to claim 1 , wherein the plant is an angiosperm.

6. The method according to claim 1 , wherein the plant is a dicotyledon.

7. The method according to claim 1 , wherein the plant is a cruciferous plant.

8. The method according to claim 1 , wherein the plant is Arabidopsis thaliana.

Priority Claims (1)
JP 2009-135321 · Jun 4, 2009 · national
Continuity (2)
Division 13376326
Related Publication 20150337322A1 · Nov 26, 2015