Method and system for sample preparation
A method for preparing a sample by utilizing a shearing force in the presence of a size stabilizer to break apart the sample to obtain nucleic acid molecules in a usable size range. Once nucleic acid molecules are obtained, magnetic nanoparticles are used to concentrate and clean the nucleic acid molecules for further testing.
1. A disposable cartridge for preparing a nucleic acid sample, the disposable cartridge comprising:
a cartridge body with an inner cylindrical surface;
a cylindrical insert disposed within the inner cylindrical surface of the cartridge body rotatably connected thereto, the cylindrical insert comprising:
a disrupting chamber fluidly connected to a first port;
at least a second chamber fluidly connected to a second port; and
first and second elongated channels disposed on opposite radial positions of a bottom edge of the cylindrical insert, each traversing at least a portion of the bottom edge of the cylindrical insert, the first elongated channel being fluidly isolated from the second elongated channel, the first elongated channel being fluidly connected to a first pair of channel ports disposed at a first radial position and the second elongated channel being fluidly connected to a second pair of channel ports disposed at a second radial position.
2. The disposable cartridge of claim 1 , wherein the first port is on an edge of the cylindrical insert.
3. The disposable cartridge of claim 1 , wherein the first port is on a vertical edge of the cylindrical insert.
4. The disposable cartridge of claim 1 , wherein the first port and the second port are at a predetermined height along a vertical edge of the cylindrical insert.
5. The disposable cartridge of claim 4 , wherein the predetermined height aligns the first port with a syringe mold on the cartridge body when the cylindrical insert is in a first rotary position and aligns the second port with the syringe mold on the cartridge body when the cylindrical insert is in a second rotary position.
6. The disposable cartridge of claim 1 , further comprising an elongated washing channel that traverses at least a portion of the bottom edge of the cylindrical insert, the elongated washing channel comprising a first portion with a first width and a second portion with a second width that is greater than the first width, the second portion providing a location to concentrate magnetic nanoparticles during a washing step.
7. The disposable cartridge of claim 1 , further comprising a column chamber.
8. The disposable cartridge of claim 7 , wherein the column chamber comprises a de-salting matrix.
9. The disposable cartridge of claim 7 , wherein the column chamber is formed by a first wall and a second wall, the second wall being shorter than first wall, the disposable cartridge further comprising an overflow chamber separated from the column chamber by the second wall.
10. The disposable cartridge of claim 1 , further comprising a barcode.
11. The disposable cartridge of claim 1 , further comprising a chip with a biological probe.
12. A system for preparing a nucleic acid sample, the system comprising
a disposable cartridge comprising:
a cartridge body with a syringe mold;
a cylindrical insert rotatably connected to the cartridge body, the cylindrical insert comprising:
a disrupting chamber fluidly connected to a first port;
at least one chamber fluidly connected to a second port;
first and second elongated channels disposed on opposite radial positions of a bottom edge of the cylindrical insert, each traversing at least a portion of the bottom edge of the cylindrical insert, the first elongated channel being fluidly isolated from the second elongated channel, the first elongated channel being fluidly connected to a first pair of channel ports disposed at a first radial position and the second elongated channel being fluidly connected to a second pair of channel ports disposed at a second radial position;
a chip with a biological probe;
a detection device comprising:
a sensor mount for receiving the chip;
a microprocessor for processing electrical signals from the chip;
a disruptor for sending ultrasonic force into the disrupting chamber;
a cartridge drive for rotating the cylindrical insert; and
a plunger drive for connecting to the syringe mold.
13. The system as recited in claim 12 , wherein the detection device further comprises a barcode reader.
14. The system as recited in claim 12 , further comprising a computer network in communication with the detection device.
15. The system as recited in claim 12 , wherein the detection device further comprises a global positioning system.
16. The system as recited in claim 12 , wherein the detection device comprises a first heater and a second heater, each independently controlled, the first heater and second heater being fixedly mounted proximate the cylindrical insert such that the cylindrical insert rotates relative to the first heater and the second heater upon actuation of the cartridge drive.
17. The system as recited in claim 12 , wherein the detection device comprises a magnet fixedly mounted proximate the cylindrical insert but offset from a center of the cylindrical insert such that the cylindrical insert rotates relative to the magnet upon actuation of the cartridge drive.
18. The system as recited in claim 12 , wherein the detection device is a portable detection device.