IP Library Granted Patent US 9,354,172
Granted Patent B2
US 9,354,172 · App. 13/437,616 · Granted May 31, 2016

Tube and reflective float systems for analyzing suspensions

Inventors: Jackie Lynn Stilwell (Sammamish, WA); Arturo Bernardo Ramirez (Seattle, WA)
Assignee: RARECYTE, INC.
G01N21/6428B01L3/50215G01N21/03B01L2300/0636B01L2300/168G01N21/645G01N2021/6439
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Quick Facts
Patent No.
US 9,354,172
App. No.
13/437,616
Granted
May 31, 2016
Kind
B2
Abstract

Tube and float systems for analyzing target materials of a suspension include in which at least a portion of the outer surface of the float is reflective are described. The target material particles can be conjugated with fluorophores. In order to identify the target material, the material between the float and tube is illuminated with one or more channels of excitation radiation, which causes the fluorophores to become excited and emit radiation at longer wavelengths. The reflective surface of the float reflects the excitation radiation and the emitted radiation.

Claims (26)

1. A system for analyzing a target material of a suspension, the system comprising:

a tube with a closed end to hold the suspension; and

a reflective float to be added to the tube, wherein the float is to trap the target material between the float and an inner wall of the tube and at least a portion of an outer surface of the float is reflective with respect to excitation light to illuminate fluorophores to be attached to particles of the target material and to light emitted from the fluorophores.

2. The system of claim 1 , wherein the at least a portion of the outer surface of the float is reflective further comprises an entire outer surface of the float is reflective.

3. The system of claim 1 , wherein the at least a portion of the outer surface of the float is reflective further comprises a main body portion of the float is reflective.

4. The system of claim 1 , wherein the at least a portion of the outer surface of the float is reflective further comprises a main body portion of the float is reflective except for the structural elements that protrude from the main body portion of the float.

5. The system of claim 1 , wherein the reflective float is composed of a reflective material.

6. The system of claim 5 , wherein the reflective material further comprises one of a metal, a ceramic, or a white pigmented polymer.

7. The system of claim 1 , wherein the at least a portion of the outer surface of the float is reflective farther comprises a reflective coating applied to the outer surface.

8. The system of claim 1 , wherein the at least a portion of the outer surface of the float is reflective further comprises a reflective plating attached to the outer surface.

9. The system of claim 1 , wherein the at least a portion of the outer surface of the float is reflective further comprises reflective particles attached to the outer surface.

10. The system of claim 1 , wherein the at least a portion of the outer surface of the float is reflective further comprises reflective particles embedded within the outer surface.

11. The system of claim 1 , wherein the reflective portion of the outer surface is functionalized to attract or attach particles of the target material to the float.

12. The system of claim 11 , wherein a self-assembled monolayer comprising a head, a tail, and a functional group is used to functionalize the reflective surface.

13. A float to be inserted into a tube with a closed end to hold a suspension, wherein the float is to trap a target material of a suspension between the float and an inner wall of the tube and the float includes a reflective outer surface to reflect excitation light used to illuminate fluorophores attached to target particles of the target material and to reflect light emitted from the fluorophores.

14. The float of claim 13 , wherein the reflective outer surface is an entire outer surface of the float.

15. The float of claim 13 , wherein the reflective outer surface is a main body portion of the float.

16. The float of claim 13 , wherein the reflective outer surface is a main body portion of the float except for structural elements that protrude from the main body portion of the float.

17. The float of claim 13 , wherein the float is composed of a reflective material.

18. The float of claim 17 , wherein the reflective material further comprises one of a metal, a ceramic, or a white pigmented polymer.

19. The float of claim 13 , wherein the reflective outer surface is a reflective coating applied to the outer surface of the float.

20. The float of claim 13 , wherein the reflective outer surface is a reflective plating attached to the outer surface of the float.

21. The float of claim 13 , wherein the reflective outer surface further comprises reflective particles attached to the outer surface of the float.

22. The float of claim 13 , wherein the reflective outer surface further comprises reflective particles embedded within the outer surface of the float.

23. The float of claim 13 , wherein the reflective outer surface is functionalized to attract or attach particles of the target material to the float.

24. The float of claim 23 , wherein a self-assembled monolayer comprising a head, a tail, and a functional group is used to functionalize the reflective outer surface.

Assignments (3)
RELEASE OF SECURITY INTEREST Recorded Dec 30, 2019
From: WESTERN ALLIANCE BANK, AN ARIZONA CORPORATION
To: RARECYTE, INC.
Reel/Frame 051445/0343 →
SECURITY INTEREST Recorded Mar 29, 2019
From: RARECYTE, INC.
To: WESTERN ALLIANCE BANK, AN ARIZONA CORPORATION
Reel/Frame 048746/0498 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Apr 2, 2012
From: RAMIREZ, ARTURO BERNARDO; STILWELL, JACKIE LYNN
To: RARECYTE, INC.
Reel/Frame 027974/0409 →
Continuity (2)
Provisional Application 61577866 · Dec 20, 2011
Related Publication 20130153784A1 · Jun 20, 2013