Bioactive carbon nanotube composite functionalized with B-sheet polypeptide block copolymer, and preparation method thereof
The present invention relates to a bioactive carbon nanotube composite functionalized with a β-sheet polypeptide block copolymer by combination self-assembly, which shows excellent water dispersion, and has biological activity so as to be used as stimulus-responsive and adaptable biomaterials or in the manufacture of CNT-based electronic biosensor devices. In addition, the bioactive carbon nanotube composite can be used as a composition for delivery of a biological active material into cells. Further, the application of the interaction between a β-sheet peptide and a carbon-based hydrophobic material is expected to be useful for designing and developing an inhibitor for diseases caused by the abnormal folding of a protein and by biomacromolecular interactions (protein-protein, protein-DNA, and protein-RNA interactions etc).
1. A hybrid of β-sheet block copolypeptides and carbon nanotubes
wherein each (β-sheet block copolypeptide consists of a β-sheet polypeptide block and a bioactive polypeptide block,
the β-sheet polypeptide block has an alternating series of nonpolar amino acids and polar amino acids or contains 50 to 100% of nonpolar amino acids,
the bioactive polypeptide block consists of polar amino acids accounting for 50-100% of the total amino acids thereof,
the β-sheet polypeptide block is non-covalently bound to the surface of the carbon nanotubes, and
the bioactive polypeptide block is charged and exposed outside the hybrid;
wherein the amino acids are the L-isomer form.
2. The hybrid of β-sheet block copolypeptides and carbon nanotubes according to claim 1 , wherein the nonpolar amino acids are selected from phenylalanine, alanine, valine, isoleucine, leucine, methionine, tyrosine, and tryptophan, and the polar amino acids are selected from lysine, glycine, arginine, proline, glutamine, serine, histidine, aspartic acid, glutamic acid, threonine, aspargine, cysteine, and selenocysteine.
3. The hybrid of β-sheet block copolypeptides and carbon nanotubes according to claim 2 , wherein the nonpolar amino acid of the β-sheet polypeptide block is phenylalanine or tryptophan, and the polar amino acid of the β-sheet polypeptide block is lysine, glutamic acid or glycine.
4. The hybrid of β-sheet block copolypeptides and carbon nanotubes according to claim 3 , wherein the β-sheet polypeptide blocks self-assemble via β-sheet hydrogen bonds among the peptide strands, and the phenylalanine or tryptophan is non-covalently bound to the surface of the carbon nanotubes via π-π stacking and hydrophobic interactions.
5. The hybrid of β-sheet block copolypeptides and carbon nanotubes according to claim 1 , wherein the β-sheet block copolypeptide is a macrocyclic peptide in which the N—and C-termini are linked to each other.
6. A biosensor comprising the hybrid of β-sheet block copolypeptides and carbon nanotubes according to any one of claims 1 to 5 , and a ligand or receptor attached to the hybrid to react with a target biomaterial.
7. The biosensor according to claim 6 , wherein the ligand or receptor is selected from enzyme substrates, ligands, amino acids, peptides, proteins, enzymes, lipids, cofactors, carbohydrates, and combinations thereof.
8. A composition comprising the hybrid of β-sheet block copolypeptides and carbon nanotubes of any of claims 1 to 5 , wherein the hybrid is localized within the cytoplasm of a cell.
9. A method for preparing a hybrid of β-sheet block copolypeptides and carbon nanotubes, the method comprising (a) removing a suspending solvent from a suspension of carbon nanotubes to obtain pretreated carbon nanotubes, and (b) adding the pretreated carbon nanotubes to an aqueous solution of β-sheet block copolypeptides to obtain a dispersion, each β-sheet block copolypeptide consisting of a β-sheet polypeptide block and a bioactive polypeptide block, the β-sheet polypeptide block having an alternating series of nonpolar amino acids and polar amino acids or containing 50 to 100% of nonpolar amino acids, the bioactive polypeptide block consisting of polar amino acids accounting for 50-100% of the total amino acids thereof, the β-sheet polypeptide block is non-covalently bound to the surface of the carbon nanotubes and the amino acids are the L-isomer form.
10. The method according to claim 9 , wherein the dispersion further comprises a salt.
11. The method according to claim 9 or 10 , wherein, in step (a), the suspending solvent is tetrahydrofuran and is removed by centrifugation.
12. The method according to claim 10 , wherein the salt is present at a concentration of 10 to 150 mM in the dispersion and the dispersion is prepared by sonication.
13. A method for internalizing peptides within the cytoplasm of a cell, the method comprising contacting a cell with a hybrid of β-sheet block copolypeptides and carbon nanotubes of any of claims 1 to 5 , wherein the bioactive polypeptide component further comprises a cell-penetrating polypeptide.
14. The composition of claim 13 , wherein the cell-penetrating polypeptide is a Tat cell-penetrating peptide.
15. The composition of claim 8 , wherein the cell is a mammalian cell.