Hand held micro PCR device including microcontroller for exchanging information with a communications interface
Instant invention is about a hand held micro PCR device comprising a LTCC micro PCR chip comprising a heater, a reaction chamber to load a sample. It also comprises a heater control to regulate the heater on basis of input received from a temperature sensor. It further has an optical system having an optical fiber to detect a fluorescence signal from the sample, and at least one communication interface to interact with other device(s).
1. A polymerase chain reaction (PCR) device comprising:
a low temperature co-fired ceramic (LTCC) PCR chip comprising:
a heater and a reaction chamber configured to hold a sample;
a plurality of thermal conductor rings surrounded by and physically separate from the reaction chamber;
a heater control interfaced with the heater;
a temperature sensor;
a temperature sensing circuit interfaced with the temperature sensor;
an optical detection system to detect a fluorescence signal from the sample;
an optical circuit interfaced with the optical detection system; and
a communication interface that connects to an external processing device that controls and monitors the PCR device.
2. The device as claimed in claim 1 , wherein the thermal conductor rings are connected to each other by a plurality of posts, the heater is embedded in a heater layer placed below a conductor layer, and the conductor layer is connected to the thermal conductor rings by a plurality of the posts.
3. The device as claimed in claim 1 , wherein the temperature sensor is placed outside the chip or embedded in at least one layer of the chip to measure temperature of the chip.
4. The device as claimed in claim 1 , wherein the chip comprises a transparent sealing cap to cover the reaction chamber.
5. The device as claimed in claim 1 , wherein the communication interface is selected from at least one of a universal serial bus (USB) interface and a Bluetooth interface.
6. The device as claimed in claim 1 , wherein the processing device is selected from the group consisting of a smart phone, a personal digital assistant (PDA), and any programmable device.
7. The device as claimed in claim 1 , further comprising:
a microcontroller electrically and communicatively coupled to the communication interface, the heater control, the temperature sensing circuit, and the optical circuit, the microcontroller programmed to:
receive input data representing a set point value from the external processing device through the communications interface,
provide the set point value to the heater control,
receive a temperature sensor value from the temperature sensing circuit,
receive the fluorescence signal from the optical circuit, and
provide the temperature sensor value and the fluorescence signal to the external processing device through the communication interface.