Monomeric VHH domain derived from anti-VP6 camelid antibodies, dimeric domain, immunisation method, composition, and treatment methods for rotavirus infections
Monomeric VHH domain derived from anti-VP6 camelid antibodies, dimeric domain, immunization method, and treatment method for rotavirus infections, wherein said domain may be any of the amino acid sequences shown in SEQ ID NO:1, SEQ ID NO:2 or SEQ ID NO:3, and wherein said domains bind to protein VP6 of Group A rotavirus.
1. A dimeric or monomeric VHH domain characterized in that said domain comprises at least one sequence selected from the group consisting of: SEQ ID NO: 1, SEQ ID NO: 2, and SEQ ID NO: 3.
2. The domain according to claim 1 , wherein the domain derives from antibodies of camelids selected from the group consisting of: Lama glama, Lama pacos, Lama guanicoe and Vicugna vicugna.
3. The domain according to claim 1 , wherein the domain binds to a linear epitope of the VP6 antigen present in group A of rotavirus.
4. The domain according to claim 1 , comprising two sequences, either identical or different, selected from the group consisting of: SEQ ID NO: 1, SEQ ID NO: 2, and SEQ ID NO: 3.
5. The domain according to claim 4 , wherein the domain further comprises a linkage of SEQ ID NO: 13.
6. The domain according to claim 4 , further comprising a sequence selected from the group consisting of: SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11 and SEQ ID NO: 12.
7. A rotavirus immunodetection method, said method comprising: a) contacting a rotavirus-containing sample with an amino acid sequence selected from the group consisting of: SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, and combinations thereof; and b) detecting the rotavirus.
8. The method in accordance with claim 7 , wherein said detecting is carried out using a technique selected from the group consisting of: immunocapture based tests ELISA, ELISPOT, competition ELISA, magnetic beads, and any other chromatographic immunoassay technology.
9. A composition designed to confer passive immunity to a mammal, the composition comprising: an amino acid sequence selected from the group consisting of: SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, and combinations thereof; excipients; and immunomodulators.
10. An antibody comprising any of the domains of claim 1 .
11. A kit for immunodetection of rotavirus comprising an amino acid sequence selected from the group consisting of: SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, and combinations thereof.
12. A method for treating infections produced by rotavirus, said method comprising administering an effective quantity of an amino acid sequence selected from the group consisting of SEQ ID NO:2 and SEQ ID NO:3, and combinations thereof, to a mammal that needs it.
13. The method of claim 12 , wherein administering comprises a route selected from the group consisting of oral, intragastric, intravenous, and intraperitoneal routes.
14. The method of claim 12 , wherein the mammal is selected from the group consisting of human beings, bovines, caprines, ovines, swines, and equines.