IP Library Granted Patent US 9,371,556
Granted Patent B2
US 9,371,556 · App. 11/073,085 · Granted Jun 21, 2016

Solutions, methods and kits for deactivating nucleic acids

Inventors: Norman C. Nelson (San Diego, CA); Kenneth A. Browne (Poway, CA); Lizhong Dai (San Diego, CA); James Russell (Vista, CA); Mark E. Filipowsky (San Marcos, CA); Margarita B. Kaminsky (San Diego, CA); Daniel L. Kacian (San Diego, CA)
Assignee: GEN-PROBE INCORPORATED
C12Q1/6802A61L2/186C11D3/3956C12Q1/6848
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Quick Facts
Patent No.
US 9,371,556
App. No.
11/073,085
Filed
Mar 4, 2005
Granted
Jun 21, 2016
Kind
B2
Art Unit
1637
USPC
435/6
Abstract

The present invention relates to reagents for use in deactivating nucleic acids and methods of making and using the same.

Claims (5)

1. A homogenous solution for use in preventing nucleic acids from acting as templates in an amplification reaction when the solution is combined with a nucleic acid deactivating agent, the solution consisting essentially of a corrosion-inhibiting agent, a wetting agent, and a solubilizing agent, each of the agents remaining substantially in solution at 22° C.,

wherein the wetting agent and the solubilizing agent are each needed to maintain the other agent substantially in solution in the presence of the corrosion-inhibiting agent,

wherein the amount of each agent is such that, when combined with a nucleic acid deactivating agent in an amount sufficient for the solution to substantially deactivate nucleic acids, the corrosion-inhibiting agent reduces the corrosive properties of the deactivating agent, the wetting agent improves the dispersion properties of the deactivating agent on a solid surface and/or increases the solubility of the deactivating agent and/or other material that may be present on a solid surface or in a solution, and the solubilizing agent increases the solubility of at least one of the deactivating agent, the corrosion-inhibiting agent and the wetting agent, and

wherein the solution does not include a nucleic acid deactivating agent;

wherein the corrosion-inhibiting agent comprises sodium bicarbonate; the wetting agent comprises a detergent; and the solubilizing agent comprises an emulsifying agent.

Assignments (8)
RELEASE OF SECURITY INTEREST Recorded Apr 28, 2026
From: BANK OF AMERICA, N.A., AS COLLATERAL AGENT
To: HOLOGIC, INC., ON ITS OWN BEHALF AND AS SUCCESSOR-BY-MERGER TO DIRECT RADIOGRAPHY CORP.; CYTYC CORPORATION, ON ITS OWN BEHALF AND AS SUCCESSOR-BY-MERGER TO BIOLUCENT, LLC; CYTYC SURGICAL PRODUCTS, LLC, AS SUCCESSOR-BY-CONVERSION TO CYTYC SURGICAL PRODUCTS, LIMITED PARTNERSHIP; GEN-PROBE INCORPORATED, ON ITS OWN BEHALF AND AS SUCCESSOR-BY-MERGER TO THIRD WAVE TECHNOLOGIES, INC.; GEN-PROBE PRODESSE, INC.; SUROS SURGICAL SYSTEMS, INC.
Reel/Frame 075566/0039 →
SECURITY INTEREST Recorded Apr 8, 2026
From: BIOTHERANOSTICS, INC.; GEN-PROBE INCORPORATED; GEN-PROBE PRODESSE, INC.; CYTYC CORPORATION; SUROS SURGICAL SYSTEMS, INC.; GYNESONICS, INC.; BOLDER SURGICAL, LLC; FAXITRON BIOPTICS, LLC; HEALTH BEACONS, INC.; HOLOGIC, INC.
To: ROYAL BANK OF CANADA, AS COLLATERAL AGENT
Reel/Frame 075462/0440 →
CORRECTIVE ASSIGNMENT TO CORRECT THE INCORRECT PATENT NO. 8081301 PREVIOUSLY RECORDED AT REEL: 035820 FRAME: 0239. ASSIGNOR(S) HEREBY CONFIRMS THE SECURITY INTEREST RELEASE. Recorded Nov 9, 2017
From: GOLDMAN SACHS BANK USA, AS COLLATERAL AGENT
To: HOLOGIC, INC.; BIOLUCENT, LLC; CYTYC CORPORATION; CYTYC SURGICAL PRODUCTS, LIMITED PARTNERSHIP; SUROS SURGICAL SYSTEMS, INC.; THIRD WAVE TECHNOLOGIES, INC.; GEN-PROBE INCORPORATED
Reel/Frame 044727/0529 →
CORRECTIVE ASSIGNMENT TO CORRECT THE INCORRECT PATENT NO. 8081301 PREVIOUSLY RECORDED AT REEL: 028810 FRAME: 0745. ASSIGNOR(S) HEREBY CONFIRMS THE SECURITY AGREEMENT. Recorded Nov 9, 2017
From: HOLOGIC, INC.; BIOLUCENT, LLC; CYTYC CORPORATION; CYTYC SURGICAL PRODUCTS, LIMITED PARTNERSHIP; SUROS SURGICAL SYSTEMS, INC.; THIRD WAVE TECHNOLOGIES, INC.; GEN-PROBE INCORPORATED
To: GOLDMAN SACHS BANK USA
Reel/Frame 044432/0565 →
SECURITY AGREEMENT Recorded Aug 7, 2015
From: HOLOGIC, INC.; BIOLUCENT, LLC; CYTYC CORPORATION; CYTYC SURGICAL PRODUCTS, LIMITED PARTNERSHIP; DIRECT RADIOGRAPHY CORP.; GEN-PROBE INCORPORATED; GEN-PROBE PRODESSE, INC.; SUROS SURGICAL SYSTEMS, INC.; THIRD WAVE TECHNOLOGIES, INC.
To: BANK OF AMERICA, N.A., AS COLLATERAL AGENT
Reel/Frame 036307/0199 →
SECURITY INTEREST RELEASE REEL/FRAME 028810/0745 Recorded Jun 4, 2015
From: GOLDMAN SACHS BANK USA, AS COLLATERAL AGENT
To: HOLOGIC, INC.; BIOLUCENT, LLC; CYTYC CORPORATION; CYTYC SURGICAL PRODUCTS, LIMITED PARTNERSHIP; SUROS SURGICAL SYSTEMS, INC.; THIRD WAVE TECHNOLOGIES, INC.; GEN-PROBE INCORPORATED
Reel/Frame 035820/0239 →
SECURITY AGREEMENT Recorded Aug 1, 2012
From: HOLOGIC, INC.; BIOLUCENT, LLC; CYTYC CORPORATION; CYTYC SURGICAL PRODUCTS, LIMITED PARTNERSHIP; SUROS SURGICAL SYSTEMS, INC.; THIRD WAVE TECHNOLOGIES, INC.; GEN-PROBE INCORPORATED
To: GOLDMAN SACHS BANK USA
Reel/Frame 028810/0745 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded May 26, 2005
From: NELSON, NORMAN C.; BROWNE, KENNETH A.; DAI, LIZHONG; RUSSELL, JAMES; FILIPOWSKY, MARK E.; KAMINSKY, MARGARITA B.; KACIAN, DANIEL
To: GEN-PROBE INCORPORATED
Reel/Frame 016278/0224 →
Continuity (2)
Provisional Application 60550749 · Mar 5, 2004
Related Publication 20050202491A1 · Sep 15, 2005