Methods and compositions for genetically detecting improved milk production traits in cattle
An isolated nucleic acid molecule comprising a polymorphic site selected from the group consisting of positions 164, 269, 284, 407 and 989 of SEQ ID NO: 1, an array or a kit comprising the same. Also provided are a method for detecting single nucleotide polymorphism (SNP) in bovine proteinase inhibitor (PI) gene, a method for haplotyping a bovine cell, a method for progeny testing of cattle based on said haplotyping, a method for selectively breeding of cattle based on haplotyping a parent animal. The present invention further provides a method for testing a dairy cattle for its milk production trait, comprising haplotyping its cells, wherein a cattle having haplotypes 1, 3, 4 or 5 indicates that the cattle has desirable milk production trait. Haplotype 1 indicates that the cattle has the most desirable milk production trait.
1. An isolated nucleic acid molecule consisting of at least 15 but not more than 50 contiguous bases of SEQ ID NO: 1, or the complement thereof, including at least a position selected from the group consisting of positions 164, 269, 284, 407 and 989 of SEQ ID NO: 1, wherein SEQ ID NO: 1 comprises:
i) an adenine base at position 164;
ii) a guanine base at position 164;
iii) a cytosine base at position 269;
iv) a thymine base at position 269;
v) a guanine base at position 284;
vi) a thymine base at position 284;
vii) a guanine base at position 407;
viii) a cytosine base at position 407;
ix) a cytosine base at position 989; or
x) a thymine base at position 989;
wherein said isolated nucleic acid molecule is labeled or is affixed to a solid support.
2. A nucleic acid molecule according to claim 1 , which comprises at least 17 contiguous bases of SEQ ID NO: 1 adjacent to the position.
3. A nucleic acid molecule according to claim 1 , which comprises at least 20 contiguous bases of SEQ ID NO: 1 adjacent to the position.
4. A nucleic acid molecule according to claim 1 , wherein the position is within 4 nucleotides of the center of the nucleic acid molecule.
5. A nucleic acid molecule according to claim 4 , wherein the position is at the center of the nucleic acid molecule.
6. A nucleic acid molecule according to claim 1 , wherein the position is at the 3-end of the nucleic acid molecule.
7. An array of nucleic acid molecules comprising at least two nucleic acid molecules according to claim 5 .
8. A kit comprising a nucleic acid molecule of claim 1 , and a suitable container.
9. A collection of isolated nucleic acid molecules according to claim 1 , said collection comprising at least two molecules each of which comprises a different position selected from the group consisting of positions 164, 269, 284, 407 and 989 of SEQ ID NO: 1.
10. The collection of isolated nucleic acid molecules according to claim 9 , comprising at least three isolated nucleic acid molecules each of which comprises a different position selected from the group consisting of positions 164, 269, 284, 407 and 989 of SEQ ID NO: 1.
11. The collection of isolated nucleic acid molecules according to claim 9 , comprising at least four isolated nucleic acid molecules each of which comprises a different position selected from the group consisting of positions 164, 269, 284, 407 and 989 of SEQ ID NO: 1.
12. The collection of isolated nucleic acid molecules according to claim 9 , comprising isolated nucleic acid molecules which collectively comprise all of positions 164, 269, 284, 407 and 989 of SEQ ID NO: 1.