Alternative splicing variant of OATP1B3 mRNA
[PROBLEM TO BE SOLVED] An object of the present invention is to provide a novel tumor marker and use thereof. In more detail, the present invention provides a novel tumor marker, a method for measuring said tumor marker and a measurement kit, a method for detecting cancer using the same, a kit for detecting cancer, a method for screening a preventive and/or therapeutic agent for cancer, as well as a medicament such as cancer vaccine. [SOLUTION] According to the present invention, a method for measuring an alternative splicing variant of OATP1B3 mRNA in a sample to be examined is provided. Said measurement method comprises measuring mRNA comprising a nucleotide sequence represented by SEQ ID NO: 1 in the sequence listing table in a sample to be examined isolated from living organism with differentiation from a mRNA comprising a nucleotide sequence represented by SEQ ID NO: 2. Said measurement method is useful for detecting cancer or screening a preventive and/or therapeutic agent.
1. An isolated deoxyribonucleic acid satisfying the following conditions:
(1) the isolated deoxyribonucleic acid is capable of hybridizing to a nucleic acid comprising the nucleotide sequence of SEQ ID NO: 1 under a stringent condition;
(2) the isolated deoxyribonucleic acid does not hybridize to a nucleic acid comprising the nucleotide sequence of SEQ ID NO: 2 under the stringent condition or all but its 3′ terminus hybridizes to the nucleic acid comprising the nucleotide sequence of SEQ ID NO: 2 under the stringent condition; and
(3) 20% to 80% of the length of the isolated deoxyribonucleic acid hybridizes to exon SV in SEQ ID NO: 1 under the stringent condition and the length of the isolated deoxyribonucleic acid that does not hybridize to exon SV under the stringent condition hybridizes to exon 3 of SEQ ID NO: 1 under the stringent condition, wherein the isolated deoxyribonucleic acid includes a fluorescent label, a biotin label, a radioactive label, or an enzyme label and is 25 to 35 nucleotides in length.
2. A kit for measuring an alternative splicing variant of OATP1B3 mRNA, comprising the isolated deoxyribonucleic acid according to claim 1 .
3. An isolated deoxyribonucleic acid having the nucleotide sequence of SEQ ID NO: 1.