Integrated analysis device for simultaneously detecting EBCs and VOCs in human exhaled breath
The present embodiments disclose a device including a module for sampling, separating and enriching a detected object, an exhaled breath condensates (EBCs) detection module and a combined volatile organic compounds (VOCs) detection module. The sampling module is connected with the EBCs detection module via a syringe pump for sample injection and is connected with the combined VOCs detection module by a capillary separation column. EBCs and VOCs in human exhaled breath are simultaneously sampled, separated and condensed; the heavy metal ions, cell factors, etc. in the collected EBCs are detected with a light addressable potentiometric sensor (LAPS); the condensed VOCs can be quantitatively detected by the combined VOCs detection module with a high sensitivity; and a heating rod and a platinum resistor can be conveniently replaced because a separated outlet heating piece is designed in the combined VOCs detection module.
1. An integrated analysis device for simultaneously detecting human exhaled breath condensates (EBCs) and volatile organic compounds (VOCs) in human exhaled breath, comprising a module for sampling, separating and enriching a detected object, an EBCs detection module and a combined VOCs detection module; the module for sampling, separating and enriching the detected object is connected with the EBCs detection module via a syringe pump for sample injection, and the module for sampling, separating and enriching the detected object is connected with the combined VOCs detection module via a capillary separation column; wherein the EBCs detection module comprises an EBC inlet ( 57 ), an inlet for washing liquid ( 58 ), a first three-way valve ( 59 ), a composite light addressable potentiometric sensor (LAPS) for heavy metal ions (Cr 3+ ) ( 60 ), a first working electrode ( 61 ), a light source controlled by a signal generating circuit ( 62 ), a reference electrode ( 63 ), a second three-way valve ( 64 ), a urea inlet ( 65 ), a detecting electrode ( 67 ), a carcinoembryonic antigen light addressable potentiometric sensor (CEA-LAPS) ( 68 ), a second working electrode ( 69 ), a Cr 3+ ion detecting cavity and a carcinoembryonic antigen (CEA) detecting cavity; the EBC inlet ( 57 ), the inlet for washing liquid ( 58 ) and the Cr 3+ ion detecting cavity are connected via the first three-way valve ( 59 ); the urea inlet ( 65 ), the Cr 3+ ion detecting cavity and the CEA detecting cavity are connected via the second three-way valve ( 64 ); the reference electrode ( 63 ) is inserted into the Cr 3+ ion detecting cavity from its top; the composite LAPS for heavy metal ions (Cr 3+ ) ( 60 ) and the first working electrode ( 61 ) are fixed to the bottom of the Cr 3+ ion detecting cavity; the first working electrode ( 61 ) is joined with the bottom of the composite LAPS for heavy metal ions (Cr 3+ ) ( 60 ); the detecting electrode ( 67 ) is inserted into the CEA detecting cavity from its top; the CEA-LAPS ( 68 ) and the second working electrode ( 69 ) are fixed to the bottom of the CEA detecting cavity, and the second working electrode ( 69 ) is joined with the bottom of the CEA-LAPS ( 68 ); at the upper portion of the CEA detecting cavity are disposed an inlet for CEA antibody-urease compound liquid ( 66 ) and an outlet for waste liquid ( 70 ); one light source controlled by the signal generating circuit ( 62 ) is placed at a position corresponding to the composite LAPS for heavy metal ions (Cr 3+ ) ( 60 ) under the Cr 3+ ion detecting cavity, and another light source controlled by the signal generating circuit ( 62 ) is placed at a position corresponding to the CEA-LAPS ( 68 ) under the CEA detecting cavity.
2. The integrated analysis device for simultaneously detecting EBCs and VOCs in human exhaled breath according to claim 1 , wherein the CEA-LAPS ( 68 ) is built by depositing a silicon dioxide (SiO 2 ) layer ( 73 ) and a silicon nitride (Si 3 N 4 ) film ( 74 ) in turn on a Si substrate ( 72 ) a chemical vapor deposition and photolithography, and forming a nanolayer ( 75 ) and a biotin layer ( 76 ) on the surface of the Si 3 N 4 film ( 74 ) by a chemical coating method.
3. The integrated analysis device for simultaneously detecting EBCs and VOCs in human exhaled breath according to claim 1 , the module for sampling, separating and enriching the detected object further comprising a mouthpiece ( 1 ), a saliva collector ( 2 ), an inspiration check valve ( 3 ), an expiration check valve ( 4 ), a first three-way solenoid valve ( 5 ), a second three-way solenoid valve ( 6 ), an activated carbon filter ( 7 ), an inlet check valve ( 8 ), a gas buffering chamber ( 9 ), a third three-way solenoid valve ( 10 ), a gas outlet ( 11 ), an adsorption tube ( 12 ), a miniature vacuum pump ( 13 ), a gas mass flow meter ( 14 ), a linear stepping motor ( 15 ), a piston ( 16 ), a washing liquid storage for condensation tube ( 17 ), a peristaltic pump ( 18 ), a condensation module ( 19 ), and a condensate collector ( 20 ); wherein the mouthpiece ( 1 ) is connected with the first three-way solenoid valve ( 5 ) via the inspiration check valve ( 3 ) and the expiration check valve ( 4 ) in turn, the saliva collector ( 2 ) is connected between the mouthpiece ( 1 ) and the inspiration check valve ( 3 ), the two outlets of the first three-way solenoid valve ( 5 ) are connected respectively with the second three-way solenoid valve ( 6 ) and the gas mass flow meter ( 14 ); the inlet check valve ( 8 ) is then connected with the third three-way solenoid valve ( 10 ) via the activated carbon filter ( 7 ), the second three-way solenoid valve ( 6 ) and the gas buffering chamber ( 9 ) in turn, the two outlets of the third three-way solenoid valve ( 10 ) is then connected respectively with the gas outlet ( 11 ) and the adsorption tube ( 12 ) and to the miniature vacuum pump ( 13 ); the gas mass flow meter ( 14 ) is connected with the condensation module ( 19 ), and all the above connections are a gas pipeline connection; the washing liquid storage for condensation tube ( 17 ) is connected with the condensate collector ( 20 ) in a liquid pipeline via the peristaltic pump ( 18 ) and the condensation module ( 19 ) in turn; the linear stepping motor ( 15 ) is connected with the piston ( 16 ), and drives the piston ( 16 ) into the condensation module ( 19 ).
4. The integrated analysis device for simultaneously detecting EBCs and VOCs in human exhaled breath according to claim 3 , the gas buffering chamber ( 9 ) further comprising a heating rod ( 21 ), a heating piece ( 22 ) and a conduit ( 23 ), wherein both the heating rod ( 21 ) and the conduit ( 23 ) are placed within the heating piece ( 22 ).
5. The integrated analysis device for simultaneously detecting EBCs and VOCs in human exhaled breath according to claim 3 , the condensation module ( 19 ) further comprising the piston ( 16 ), a gas inlet ( 26 ), an inlet for washing liquid ( 27 ), a condensation tube ( 28 ), an ice cooling box ( 29 ), and a condensate outlet ( 30 ); the gas inlet ( 26 ), the inlet for washing liquid ( 27 ), the condensate outlet ( 30 ) and a condensation tube ( 28 ) are formed integrally, the piston ( 16 ) is inserted into the condensation tube ( 28 ), and the ice cooling box ( 29 ) is in close contact with the outside of the condensation tube ( 28 ).
6. The integrated analysis device for simultaneously detecting EBCs and VOCs in human exhaled breath according to claim 3 , the module for sampling, separating and enriching the detected object further comprising an aluminium piece ( 24 ) and a cooling plate ( 25 ), the aluminium piece ( 24 ) is in close contact with the periphery of the adsorption tube ( 12 ), and the outside of the aluminium piece ( 24 ) is fixed with the semiconductor cooling plate ( 25 ).
7. The integrated analysis device for simultaneously detecting EBCs and VOCs in human exhaled breath according to claim 1 , the combined VOCs detection module further comprising an outlet heating piece ( 31 ), a gas nozzle ( 32 ), a upper cover of sensor gaschamber ( 33 ), a heat sink ( 34 ), and a conduit for capillary extraction ( 35 ); the gas nozzle ( 32 ) and the conduit for capillary extraction ( 35 ) are respectively in a direct threaded connection with the outlet heating piece ( 31 ), the upper cover of sensor gas-chamber ( 33 ) is snap-fitted with the heat sink ( 34 ), the upper cover of sensor gas-chamber ( 33 ) is connected with the outlet heating piece ( 31 ), and the gas nozzle ( 32 ) extends into the upper cover of sensor gaschamber ( 33 ).
8. The integrated analysis device for simultaneously detecting EBCs and VOCs in human exhaled breath according to claim 7 , the outlet heating piece ( 31 ) further comprising a cambered guiding groove for capillary ( 41 ), a slot for heating rod ( 45 ), a slot for platinum resistor ( 46 ), a first insertion screw hole ( 47 ), and a second insertion screw hole ( 48 ), a heating rod and a platinum resistor; the two ends of the cambered guiding groove for capillary ( 41 ) are respectively connected with the first insertion screw hole ( 47 ) and the second insertion screw hole ( 48 ), and the heating rod and the platinum resistor are respectively inserted into the slot for heating rod ( 45 ) and the slot for platinum resistor ( 46 ).