Nanostructured devices for detecting and analyzing biomolecules
A biosensing FET device, comprising a plurality of nanostructured SOI channels, that is adapted to operate in solutions having a high ionic strength and provides improves sensitivity and detection. Generally, the biosensing device comprises an underlying substrate layer, an insulator and a semiconductor layer and a plurality of channels in the semiconductor layer comprising a plurality of whole or partially formed nanopores in the channels.
1. A biosensing FET device having a sensing surface, comprising:
a semiconductor layer comprising one or more channels having one or more nanopores in the channel, wherein the nanopores comprise a biomolecule;
an insulator layer;
an underlying substrate layer; and wherein the biosensing FET device is adapted to operate in solutions having an ionic strength that is equal to or less than 200 mM.
2. The biosensing device of claim 1 , wherein one or more of said channels has a height and one or more of said nanopores has a depth that is less than the height of one or more of said channels.
3. The biosensing device of claim 1 , wherein one or more of the channels has a height, and wherein one or more of the nanopores in the channels has a depth that is the same as the height of one or more of the channels.
4. The biosensing device of claim 3 , wherein the nanopores have a depth that is greater than the height of one or more of the channels so that the nanopores extend through the top surface of, and partially into, the insulator layer, and wherein the nanopores have an inside surface, at least a portion of which is functionalized, that is partially in the semiconductor layer and partially in the insulator layer.
5. The biosensing device of claim 4 , wherein the nanopores are functionalized, at least in part, with one or more binders.
6. The biosensing device of claim 1 , wherein one or more of the nanopores is functionalized, at least in part, with one or more binders.
7. The biosensing device of claim 1 wherein the nanopores in the channel of the semiconductor layer locate one or more binding sites proximate the sensing surface.
8. The biosensing device of claim 7 , is adapted to operate in solutions having an ionic strength that is equal to or less than 10 mM, and wherein the nanopores locate one or more of the binding sites less than or equal to 5 nm from the sensing surface.
9. The biosensing device of claim 7 , wherein the nanopores locate the binding sites less than or equal to 1 nm from the sensing surface.
10. The biosensing device of claim 1 , wherein the channels have a density of nanopores that is between 10 10 to 10 12 per cm −2 .
11. The biosensing device of claim 1 , wherein the semiconductor layer comprises silicon.
12. A biodetector comprising the biosensing device of claim 1 .