Antibody composition-producing cell
The present invention relates to a cell for the production of an antibody molecule such as an antibody useful for various diseases having high antibody-dependant cell-mediated cytotoxic activity, a fragment of the antibody and a fusion protein having the Fc region of the antibody or the like, a method for producing an antibody composition using the cell, the antibody composition and use thereof.
1. An animal cell containing a DNA encoding an antibody, wherein said animal cell is modified, by a modification, to exhibit a reduced amount, or an absence, of N-glycoside-linked sugar chains containing fucose linked through an α-1,6 linkage, and wherein said animal cell produces antibodies that do not contain fucose linked through an α-1,6 linkage,
wherein said modification causes a reduction in, or an absence of, at least one intracellular sugar nucleotide in said cell, said at least one intracellular sugar nucleotide being selected from the group consisting of: GDP-α4-keto-6-deoxymannose; GDP-α4-keto-6-deoxygalactose; and GDP-fucose.
2. The animal cell according to claim 1 , wherein said modification is the introduction into said cell, or the deletion from said cell, of an enzyme, said introduction or deletion causing a reduction in the amount, or an absence, of the at least one intracellular sugar nucleotide in said cell.
3. The animal cell according to claim 2 , wherein the enzyme is an enzyme which alters the structure of a substrate for an enzyme in a GDP-fucose synthesis pathway.
4. The animal cell according to claim 3 , wherein the enzyme in the GDP-fucose synthesis pathway is GDP-keto-6-deoxymannose 3,5-epimerase 4-reductase.
5. The animal cell according to claim 1 , wherein the antibodies produced by said cell that do not contain fucose linked through an α-1,6 linkage constitute 20% or more of the total antibodies produced by said cell.
6. The animal cell according to claim 5 , wherein the antibodies produced by said cell that do not contain fucose linked through an α-1,6 linkage constitute 50% or more of the total antibodies produced by said cell.
7. The animal cell according to claim 1 , wherein the animal cell is a CHO cell.