Method of reseeding adherent cells grown in a hollow fiber bioreactor system
This invention is directed to methods of directly reseeding harvested adherent cells grown in a hollow fiber bioreactor. Also disclosed is a novel harvest media for use in directly reseeding adherent cells into a hollow fiber bioreactor.
1. A media for harvesting cells grown in a hollow fiber bioreactor, the media comprising:
base media;
protein;
3.9 mM calcium chloride;
9.91 mM anhydrous D-glucose; and
2.24 mM magnesium sulfate.
2. The media of claim 1 , wherein the base media comprises alpha-MEM.
3. The media of claim 1 , wherein the protein comprises one or more from the group consisting of: human platelet lysate, human plasma, fetal bovine serum, and fetal calf serum.
4. The media of claim 1 , wherein the media is used to compensate for a loss of metabolites through hollow fibers of the hollow fiber bioreactor during a harvest procedure.