DNA/RNA in a Y-shaped nanochannel
View Patent ↗A mechanism is provided for ratcheting a double strand molecule. The double strand molecule is driven into a Y-channel of a membrane by a first voltage pulse. The Y-channel includes a stem and branches, and the branches are connected to the stem at a junction. The double strand molecule is slowed at the junction of the Y-channel based on the first voltage pulse being weaker than a force required to break a base pair of the double strand molecule. The double strand molecule is split into a first single strand and a second single strand by driving the double strand molecule into the junction of the Y-channel at a second voltage pulse.
1. A system for ratcheting a double strand molecule, the system comprising:
a membrane comprising a first Y-channel, the first Y-channel comprises a first stem, a first left branch, and a first right branch formed in the membrane, the first left and right branches being connected to the first stem at a first junction;
a second Y-channel comprising a second stem, a second left branch, and a second right branch formed in the membrane, the second left and right branches being connected to the second stem at a second junction, wherein the second stem of the second Y-channel is connected to the first left branch of the first Y-channel such that the first left branch feeds into the second Y-channel, wherein the second junction comprises a sharp end configured to separate base pairs, wherein the first stem comprises a diameter of 4-10 nm, the first left branch comprises a diameter of 2-4 nm, the first right branch comprises a diameter of 2-4 nm, and the sharp end of the second junction comprises an angle of 30-120 degrees;
a top fluidic chamber on one side of the membrane and a bottom fluidic chamber on an opposing side of the membrane, such that the first and second Y-channels communicate with both the top fluidic chamber and the bottom fluidic chamber.
2. The system of claim 1 , wherein the double strand molecule is deoxyribonucleic acid or ribonucleic acid.
3. The system of claim 1 , wherein the membrane comprises three or more Y-channels, each having a stem and corresponding branches.
4. The system of claim 1 , wherein the first and second Y-channels are Y-shaped carbon nanotubes.
5. The system of claim 1 , wherein a voltage source is configured to apply a first voltage pulse, the first voltage pulse being configured to drive the double strand molecule into the first and second Y-channels of the membrane.
6. The system of claim 1 , wherein the first stem has a diameter greater than each of the first left and right branches;
wherein the first left and right branches each have a diameter greater than the second stem of the second Y-channel; and
wherein the second stem has a diameter greater than each of the second left and right branches.
7. The system of claim 1 , further comprising a third Y-channel comprising a third stem, a third left branch, and a third right branch formed in the membrane, the second left and right branches being connected to the third stem at a third junction, wherein the third stem of the third Y-channel is connected to the first right branch of the first Y-channel such that the first right branch feeds into the third Y-channel.
8. The system of claim 1 , wherein a difference in diameter from the second stem to the second left and right branches is about 1 nm.
9. The system of claim 8 , wherein a difference in diameter from the first stem to the second stem is about 2 nm.