Phosphatidic acid phosphatase gene
The present invention provides a novel phosphatidic acid phosphatase gene. The object of the present invention can be solved by providing a nucleic acid comprising the nucleotide sequence set forth in SEQ ID NO: 1, SEQ ID NO: 4, or SEQ ID NO: 5; a protein comprising the amino acid sequence set forth in SEQ ID NO: 2; and mutants thereof.
1. A nucleic acid comprising:
(a) a nucleotide sequence that has an identity of 95% or more with the nucleotide sequence set forth in SEQ ID NO: 1 and that encodes a protein having the ability to catalyze the reaction of generating diacylglycerol by dephosphorylation of phosphatidic acid;
(b) an intron-free nucleotide sequence encoding a protein that consists of an amino acid sequence having an identity of 95% or more with the amino acid sequence set forth in SEQ ID NO: 2 and that has the ability to catalyze the reaction of generating diacylglycerol by dephosphorylation of phosphatidic acid;
(c) a nucleotide sequence that has an identity of 95% or more with the nucleotide sequence set forth in SEQ ID NO: 3 and that encodes a protein having the ability to catalyze the reaction of generating diacylglycerol by dephosphorylation of phosphatidic acid; or
(d) a nucleotide sequence that has an identity of 95% or more with the nucleotide sequence set forth in SEQ ID NO: 5 and that encodes a protein having the ability to catalyze the reaction of generating diacylglycerol by dephosphorylation of phosphatidic acid.
2. A cDNA comprising a nucleic acid according to any one of (a)-(e) below:
(a) a nucleotide sequence encoding a protein that consists of an amino acid sequence with deletion, substitution, or addition of 1 to 15 amino acids in the amino acid sequence set forth in SEQ ID NO: 2 and that has the ability to catalyze the reaction of generating diacylglycerol by dephosphorylation of phosphatidic acid;
(b) a nucleotide sequence that has an identity of 95% or more with the nucleotide sequence set forth in SEQ ID NO: 1 and that encodes a protein having the ability to catalyze the reaction of generating diacylglycerol by dephosphorylation of phosphatidic acid;
(c) a nucleotide sequence encoding a protein that consists of an amino acid sequence having an identity of 95% or more with the amino acid sequence set forth in SEQ ID NO: 2 and that has the ability to catalyze the reaction of generating diacylglycerol by dephosphorylation of phosphatidic acid;
(d) a nucleotide sequence that has an identity of 95% or more with the nucleotide sequence set forth in SEQ ID NO: 3 and that encodes a protein having the ability to catalyze the reaction of generating diacylglycerol by dephosphorylation of phosphatidic acid; or (e) a nucleotide sequence that has an identity of 95% or more with the nucleotide sequence set forth in SEQ ID NO: 5 and that encodes a protein having the ability to catalyze the reaction of generating diacylglycerol by dephosphorylation of phosphatidic acid.
3. The nucleic acid according to claim 1 , wherein the nucleic acid comprises:
(a) the nucleotide sequence set forth in SEQ ID NO: 1;
(b) the nucleotide sequence set forth in SEQ ID NO: 3; or
(c) the nucleotide sequence set forth in SEQ ID NO: 5.
4. The nucleic acid according to claim 1 , wherein the ability to catalyze the reaction of generating diacylglycerol by dephosphorylation of phosphatidic acid has a higher substrate specificity for a C 18 acyl group-containing phosphatidic acid than for a C 17 acyl group-containing one.
5. The cDNA according to claim 2 , wherein the ability to catalyze the reaction of generating diacylglycerol by dephosphorylation of phosphatidic acid has a higher substrate specificity for a C 18 acyl group-containing phosphatidic acid than for a C 17 acyl group-containing one.
6. A recombinant vector comprising the nucleic acid according to claim 1 .
7. A transformant transformed with the recombinant vector according to claim 6 , wherein the transformant is a host cell selected from a bacterial cell, a fungal cell, an isolated plant cell, or an isolated animal cell.
8. A recombinant vector comprising the cDNA according to claim 2 .
9. A transformant transformed with the recombinant vector according to claim 8 , wherein the transformant is a host cell selected from a bacterial cell, a fungal cell, an isolated plant cell, or an isolated animal cell.