Regulatory polynucleotides and uses thereof
The present disclosure provides compositions and methods for regulating expression of transcribable polynucleotides in plant cells, plant tissues, and plants. Compositions include regulatory polynucleotide molecules capable of providing expression in plant tissues and plants. Methods for expressing polynucleotides in a plant cell, plant tissue, or plants using the regulatory polynucleotide molecules disclosed herein are also provided.
1. A regulatory polynucleotide
comprising a nucleic acid molecule of SEQ ID NOs: 9 wherein the nucleic acid molecule is capable of regulating transcription of an operably linked transcribable polynucleotide molecule.
2. A recombinant polynucleotide construct comprising the regulatory polynucleotide of claim 1 operably linked to a transcribable polynucleotide molecule.
3. The recombinant polynucleotide construct of claim 2 , wherein the transcribable polynucleotide molecule encodes a protein of agronomic interest.
4. The recombinant polynucleotide construct of claim 2 , wherein the transcribable polynucleotide molecule is operably linked to a 3′ transcription termination polynucleotide molecule.
5. A chimeric polynucleotide molecule comprising the regulatory polynucleotide of claim 1 operably linked to
a second polynucleotide molecule capable of regulating transcription of an operably linked polynucleotide molecule.
6. The chimeric polynucleotide of claim 5 , wherein the second polynucleotide molecule is selected from the group consisting of a cis-element, an enhancer element, and an intron.
7. The chimeric polynucleotide of claim 6 , wherein the second polynucleotide molecule comprises an intron.
8. A transgenic host cell comprising the recombinant polynucleotide construct of claim 2 .
9. The transgenic host cell of claim 8 , wherein the host cell is a plant cell.
10. A transgenic plant stably transformed with the recombinant polynucleotide construct of claim 2 .
11. The transgenic plant of claim 10 , wherein the plant is selected from the group consisting of a monocotyledonous and a dicotyledonous plant.
12. The transgenic plant of claim 11 , wherein the plant is a monocotyledonous plant selected from the group consisting of wheat, corn, rice, turf grass, millet, sorghum, switchgrass, miscanthus, sugarcane, and Brachypodium.
13. transgenic plant of claim 11 , wherein the plant is a dicotyledonous plant selected from the group consisting of soybean, cotton, canola, and potato.
14. A seed produced by the transgenic plant of claim 10 , wherein said seed comprises said recombinant polynucleotide construct.
15. A method of directing expression of a transcribable polynucleotide molecule in a host cell comprising:
(a) introducing the recombinant polynucleotide construct of claim 2 into a host cell to produce a transgenic host cell; and
(b) selecting a transgenic host cell exhibiting expression of the transcribable polynucleotide molecule.
16. The method of claim 15 , wherein the transcribable polynucleotide molecule is selected from the group consisting of a coding sequence and a functional RNA.
17. The method of claim 15 , wherein the host cell is a plant cell.
18. The method of claim 17 , further comprising regenerating a plant comprising the introduced recombinant polynucleotide construct.
19. A method of directing expression of a transcribable polynucleotide molecule in a plant comprising:
(a) introducing the recombinant polynucleotide construct of claim 2 into a plant cell;
(b) regenerating a plant from the plant cell; and
(c) selecting a transgenic plant exhibiting expression of the transcribable polynucleotide molecule.
20. The method of claim 19 , wherein the transcribable polynucleotide molecule is selected from the group consisting of a coding sequence and a functional RNA.