Methods and compositions for producing aluminum tolerant alfalfa
The invention relates to alfalfa plants and lines having aluminum tolerance. The invention also relates to parts of alfalfa plants from lines having aluminum tolerance, including seeds capable of growing aluminum tolerant alfalfa plants. Methods for the use and breeding of aluminum tolerant alfalfa plants are also provided.
1. A method for increasing the aluminum tolerance of an alfalfa line, said method comprising introgressing at least one chromosomal locus contributing to aluminum tolerance from a parent alfalfa plant into a selected alfalfa line, wherein said chromosomal locus maps between loci Mstri9857-18793A97 and AW260-24554 on linkage group 4.
2. The method of claim 1 , wherein the aluminum tolerant alfalfa plant is an agronomically elite plant.
3. The method of claim 1 , wherein the aluminum tolerant alfalfa plant is a hybrid or inbred plant.
4. The method of claim 1 , wherein the introgressing is by marker-assisted selection using at least a first genetic marker linked to said chromosomal locus.
5. The method of claim 4 , wherein the marker is selected from one of those detectable using a primer pair in Table 1.
6. The method of claim 1 , wherein the parent alfalfa plant is Altet-4.
7. The method of claim 1 , wherein the parent alfalfa plant is a Medicago sativa NECS-141 plant.
8. The method of claim 1 , wherein the parent alfalfa plant exhibits at least a 50% reduction in aluminum sensitivity relative to the less aluminum tolerant alfalfa line.
9. The method of claim 8 , wherein the parent alfalfa plant displays at least a 75% reduction in aluminum sensitivity relative to the less aluminum tolerant alfalfa line.
10. The method of claim 1 further comprising producing an alfalfa seed by crossing the aluminum tolerant plant with itself or a second alfalfa plant and allowing seed to form.
11. A method for obtaining an alfalfa plant comprising an allele conferring aluminum tolerance, said method comprising:
a) obtaining nucleic acids from an alfalfa plant comprising at least a first allele that confers aluminum tolerance, wherein said allele maps between loci Mstri9857-18793A97 and AW260-24554 on linkage group 4;
b) assaying said nucleic acids for the presence of at least a first genetic marker that is genetically linked to said allele; and
c) selecting the alfalfa plant based on the presence of said genetic marker.
12. The method of claim 11 , wherein the alfalfa plant is a progeny of a plant of Altet-4.
13. The method of claim 11 , wherein the alfalfa plant is a progeny of a plant of a Medicago sativa NECS-141 plant.