Methods of renal cancer detection
Disclosed are methods for detecting, diagnosing or monitoring a renal cancer in a subject. The methods include detecting quantity of one or more polypeptides or fragments thereof comprised by body fluid such as urine, wherein the one or more polypeptides or fragments thereof, can be present at elevated levels in a subject with, a kidney cancer, as compared to a subject without a kidney cancer. Non-limiting examples of such polypeptides include aquaporin-1, adipose differentiation-related protein, and paired box protein-2. Antibody probes can be used to detect or quantify the polypeptides. In some embodiments, mass spectroscopy can be used to detect or quantify the polypeptides, or to identify a polypeptide in a body fluid sample from a subject with a kidney cancer.
1. A method of detecting a renal cancer selected from the group consisting of a clear cell carcinoma, a papillary carcinoma and a combination thereof in a subject, the method comprising:
providing a urine sample from a subject suspected of having a renal cancer selected from the group consisting of a clear cell carcinoma, a papillary carcinoma and a combination thereof;
contacting the urine sample with at least one antibody that binds adipose differentiation-related protein (ADFP), under conditions sufficient for formation of a primary complex comprising the at least one antibody and the ADFP if present;
measuring quantity of the primary complex;
comparing the quantity of the primary complex to that of a control complex formed from the at least one antibody and a urine sample of an individual who does not have a renal cancer selected from the group consisting of a clear cell carcinoma, a papillary carcinoma and a combination thereof; and
detecting a renal cancer selected from the group consisting of a clear cell carcinoma, a papillary carcinoma and a combination thereof in the subject if the primary complex comprises the ADFP at a statistically significant elevated level compared to the control complex.
2. A method of detecting a renal cancer selected from the group consisting of a clear cell carcinoma, a papillary carcinoma and a combination thereof in accordance with claim 1 , wherein the at least one antibody is a monoclonal antibody.
3. A method of detecting a renal cancer selected from the group consisting of a clear cell carcinoma, a papillary carcinoma and a combination thereof in accordance with claim 1 , wherein the detecting comprises an immunoprecipitation assay, an ELISA, a radioimmunoassay, a Western blot assay, a dip stick assay, or a bead assay.
4. A method of detecting a renal cancer selected from the group consisting of a clear cell carcinoma, a papillary carcinoma and a combination thereof in accordance with claim 1 , wherein the contacting comprises:
a) contacting the urine sample with a solid surface that binds the ADFP if present; and
b) subsequent to a), contacting the surface with the at least one antibody.
5. A method of detecting a renal cancer selected from the group consisting of a clear cell carcinoma, a papillary carcinoma and a combination thereof in accordance with claim 1 , wherein the at least one antibody is bound to a polystyrene bead.
6. A method of detecting a renal cancer selected from the group consisting of a clear cell carcinoma, a papillary carcinoma and a combination thereof in accordance with claim 1 , further comprising:
contacting a control urine sample from a person not suspected of having a renal cancer selected from the group consisting of a clear cell carcinoma, a papillary carcinoma and a combination thereof with the at least one antibody that binds ADFP under conditions sufficient for formation of a control complex comprising the at least one antibody and the ADFP if present; and
measuring quantity of the control complex.