IP Library Granted Patent US 9,505,767
Granted Patent B2
US 9,505,767 · App. 14/477,566 · Granted Nov 29, 2016

Pyrazolo[1,5-A]pyrimidin-7(4H)-onehistone demethylase inhibitors

Inventors: Brian K. Albrecht (Cambridge, MA); Steven F. Bellon (Wellesley, MA); Victor S. Gehling (Somerville, MA); Jean-Christophe Harmange (Andover, MA); Yves LeBlanc (Kirkland, CA); Jun Liang (South San Francisco, CA); Steven R. Magnuson (South San Francisco, CA); Vickie Hsiao-Wei Tsui (South San Francisco, CA); Birong Zhang (South San Francisco, CA)
Assignees: Genentech, Inc.; Constellation Pharmaceuticals, Inc.
C07D487/04A61K31/519A61K45/06A61N5/10
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Quick Facts
Patent No.
US 9,505,767
App. No.
14/477,566
Granted
Nov 29, 2016
Kind
B2
Abstract

The present invention relates to compounds formula (I): and to salts thereof, wherein R 1 -R 4 and A have any of the values defined in the specification, and compositions and uses thereof. The compounds are useful as inhibitors of histone demethylases, such as KDM5. Also included are pharmaceutically acceptable compositions comprising the compounds of the present invention and methods of using said compositions in the treatment of various disorders.

Claims (34)

1. A compound of formula (I):

wherein:

R 1 and R 2 are each independently H, C 1-12 alkyl, C 2-12 alkenyl, C 2-12 alkynyl, carbocyclyl, heterocyclyl, halo, —OR a , —SR a , —N(R a ) 2 , —CN, —NO 2 , —C(O)R a , —CO 2 R a , —C(O)N(R a ) 2 , —C(O)SR a , —C(O)C(O)R a , —C(O)CH 2 C(O)R a , —C(S)N(R a ) 2 , —C(S)OR a , —S(O)R a , —SO 2 R a , —SO 2 N(R a ) 2 , —N(R a )C(O)R a , —N(R a )C(O)N(R a ) 2 , —N(R a )SO 2 R a , —N(R a )SO 2 N(R a ) 2 , —N(R a )N(R a ) 2 , —N(R a )C(═N(R a ))N(R a ) 2 , —C═NOR a , —C(═N(R a ))N(R a ) 2 , —OC(O)R a , or —OC(O)N(R a ) 2 , wherein each C 1-12 alkyl, C 2-12 alkenyl, C 2-12 alkynyl, carbocyclyl, and heterocyclyl of R 1 and R 2 is independently optionally substituted with one or more groups R x ; and wherein R 1 and R 2 are not each H; or R 1 and R 2 taken together with the atoms to which they are attached form a 4, 5, 6, 7, or 8 membered carbocyclyl, which carbocyclyl is optionally substituted with one or more groups R x ;

R 3 is H, C 1-6 alkyl, trifluoromethyl, 3-6 membered carbocyclyl, 3-6 membered heterocyclyl, halo, —OR f , —SR f , —N(R f ) 2 , —CN, or —NO 2 , wherein said alkyl, carbocyclyl and heterocyclyl are optionally substituted with one or more groups independently selected from oxo, halo, C 1-3 alkoxy and C 1-3 alkyl;

R 4 is a pyrimidine, thiazole, pyridine, isoquinoline, or pyridazine ring, which ring is optionally substituted with one or more groups independently selected from C 1-6 alkyl, carbocyclyl, —F, —Cl, —Br, —I, —N(R v ) 2 , —O—R v , and —C(O)—O—R v ;

R 5 is H, C 1-12 alkyl, C 2-12 alkenyl, C 2-12 alkynyl, carbocyclyl, and heterocyclyl, wherein each C 1-12 alkyl, C 2-12 alkenyl, C 2-12 alkynyl, carbocyclyl, and heterocyclyl is optionally substituted with one or more groups independently selected from oxo, C 1-12 alkyl, C 1-12 haloalkyl, carbocyclyl, heterocyclyl, halo, —CN, —NO 2 , —NR m R m , —OR m , —C(═O)OR m , and —OC(═O)R m ; or R 5 and R 2 taken together with the atoms to which they are attached form a heterocyclyl;

each R a is independently selected from H, C 1-6 alkyl, C 2-6 alkenyl, C 2-6 alkynyl, carbocyclyl, and heterocyclyl, wherein each C 1-6 alkyl, C 2-6 alkenyl, C 2-6 alkynyl, carbocyclyl, and heterocyclyl is optionally substituted with one or more groups R x ;

each R f is independently selected from H, C 1-3 alkyl, trifluoromethyl, 3-6 membered carbocyclyl, and 3-6 membered heterocyclyl; or two R f groups together with the nitrogen to which they are attached form a 3-6 membered heterocycle;

each R g is independently selected from H, C 1-6 alkyl, C 2-6 alkenyl, C 2-6 alkynyl, C 3-8 carbocyclyl, and heterocyclyl, wherein each C 1-6 alkyl, C 2-6 alkenyl, C 2-6 alkynyl, C 3-8 carbocyclyl, and heterocyclyl is optionally substituted with one or more groups R x ; or two R g groups together with the nitrogen to which they are attached form a 3-6 membered heterocycle;

each R m is independently selected from H, C 1-6 alkyl, C 2-6 alkenyl, C 2-6 alkynyl, C 1-6 haloalkyl, carbocyclyl, C 1-6 alkanoyl, phenyl, and benzyl, wherein any C 1-6 alkyl, C 2-6 alkenyl, C 2-6 alkynyl, C 1-6 haloalkyl, carbocyclyl, C 1-6 alkanoyl, phenyl, or benzyl is optionally substituted with one or more groups independently selected from halo, —CN, —NO 2 , —NR y R z , and —OR w ; or two R m groups together with the nitrogen to which they are attached form a 3-6 membered heterocycle;

A is a pyrazole, imidazole, oxadiazole, or isoxazole ring, which pyrazole, imidazole, oxadiazole, or isoxazole ring is substituted with R 4 and which pyrazole, imidazole, oxadiazole, or isoxazole ring is also optionally substituted with one or more groups independently selected from halo, nitro, cyano, C 1-6 alkyl, C 2-6 alkenyl, C 2-6 alkynyl, C 1-6 haloalkyl, C 1-6 alkoxy, C 1-6 alkanoyl, C 1-6 alkanoyloxy, C 1-6 alkoxycarbonyl, carbocyclyl, and —N(R t ) 2 ;

each R t is independently selected from H, C 1-6 alkyl, C 2-6 alkenyl, C 2-6 alkynyl, C 3-8 carbocyclyl, and heterocyclyl, wherein each C 1-6 alkyl, C 2-6 alkenyl, C 2-6 alkynyl, C 3-8 carbocyclyl, and heterocyclyl is optionally substituted with one or more groups R x ; or two R t groups together with the nitrogen to which they are attached form a 3-6 membered heterocycle;

each R v is independently hydrogen, C 1-6 alkyl, C 2-6 alkenyl, C 2-6 alkynyl, carbocyclyl, and heterocyclyl, wherein each C 1-6 alkyl, C 2-6 alkenyl, C 2-6 alkynyl, carbocyclyl, and heterocyclyl is optionally substituted with one or more groups independently selected from oxo, halo, amino, hydroxyl, and C 1 -C 6 alkyl that is optionally substituted with one or more groups independently selected from oxo and halo; or two R v are taken together with the nitrogen to which they are attached to form a heterocyclyl that is optionally substituted with one or more groups independently selected from oxo, halo and C 1-3 alkyl that is optionally substituted with one or more groups independently selected from oxo and halo;

each R w is independently selected from H, C 1-4 alkyl, C 1-4 alkanoyl, phenyl, benzyl, and phenethyl;

each R x is independently selected from oxo, C 1-6 alkyl, C 2-6 alkenyl, C 2-6 alkynyl, C 1-6 haloalkyl, carbocyclyl, —F, —Cl, —Br, —I, —NO 2 , —N(R v ) 2 , —CN, —C(O)—N(R v ) 2 , —S(O)—N(R v ) 2 , —S(O) 2 —N(R v ) 2 , —O—R v , —S—R v , —O—C(O)—R v , —O—C(O)—O—R v , —C(O)—R v , —C(O)—O—R v , —S(O)—R v , —S(O) 2 —R v , —O—C(O)—N(R v ) 2 , —N(R v )—C(O)—OR v , —N(R v )—C(O)—N(R v ) 2 , —S(O) 2 —N(R v ) 2 , —N(R v )—C(O)—R v , —N(R v )—S(O)—R v , —N(R v )—S(O) 2 —R v , —N(R v )—S(O)—N(R v ) 2 , and —N(R v )—S(O) 2 —N(R v ) 2 , wherein any C 1-6 alkyl, C 2-6 alkenyl, C 2-6 alkynyl, C 1-6 haloalkyl and carbocyclyl is optionally substituted with one or more groups independently selected from oxo, halo, —NO 2 , —N(R v ) 2 , —CN,

—C(O)—N(R v ) 2 , —S(O)—N(R v ) 2 , —S(O) 2 —N(R v ) 2 , —O—R v , —S—R v , —O—C(O)—R v , —C(O)—R v , —C(O)—O—R v , —S(O)—R v , —S(O) 2 —R v , —C(O)—N(R v ) 2 , —S(O) 2 —N(R v ) 2 , —N(R v )—C(O)—R v , —N(R v )—S(O)—R v —N(R v )—S(O) 2 —R v and C 1-6 alkyl that is optionally substituted with one or more groups independently selected from oxo and halo; and

each R y and R z is independently selected from H, C 1-4 alkyl, C 1-4 alkanoyl, C 1-4 alkoxycarbonyl, phenyl, benzyl, and phenethyl, or R y and R z together with the nitrogen to which they are attached form a heterocyclyl; or a salt thereof.

2. A compound of formula (I):

wherein:

R 1 and R 2 are each independently H, C 1-12 alkyl, C 2-12 alkenyl, C 2-12 alkynyl, carbocyclyl, heterocyclyl, halo, —OR a , —SR a , —N(R a ) 2 , —CN, —NO 2 , —C(O)R a , —CO 2 R a , —C(O)N(R a ) 2 , —C(O)SR a , —C(O)C(O)R a , —C(O)CH 2 C(O)R a , —C(S)N(R a ) 2 , —C(S)OR a , —S(O)R a , —SO 2 R a , —SO 2 N(R a ) 2 , —N(R a )C(O)R a , —N(R a )C(O)N(R a ) 2 , —N(R a )SO 2 R a , —N(R a )SO 2 N(R a ) 2 , —N(R a )N(R a ) 2 , —N(R a )C(═N(R a ))N(R a ) 2 , —C═NOR a , —C(═N(R a ))N(R a ) 2 , —OC(O)R a , or —OC(O)N(R a ) 2 , wherein each C 1-12 alkyl, C 2-12 alkenyl, C 2-12 alkynyl, carbocyclyl, and heterocyclyl of R 1 and R 2 is independently optionally substituted with one or more groups R x ; and wherein R 1 and R 2 are not each H; or R 1 and R 2 taken together with the atoms to which they are attached form a 4, 5, 6, 7, or 8 membered carbocyclyl, which carbocyclyl is optionally substituted with one or more groups R x ;

R 3 is H, C 1-6 alkyl, trifluoromethyl, 3-6 membered carbocyclyl, 3-6 membered heterocyclyl, halo, —OR f , —SR f , —N(R f ) 2 , —CN, or —NO 2 , wherein said alkyl, carbocyclyl and heterocyclyl are optionally substituted with one or more groups independently selected from oxo, halo, C 1-3 alkoxy and C 1-3 alkyl;

R 5 is H, C 1-12 alkyl, C 2-12 alkenyl, C 2-12 alkynyl, carbocyclyl, and heterocyclyl, wherein each C 1-12 alkyl, C 2-12 alkenyl, C 2-12 alkynyl, carbocyclyl, and heterocyclyl is optionally substituted with one or more groups independently selected from oxo, C 1-12 alkyl, C 1-12 haloalkyl, carbocyclyl, heterocyclyl, halo, —CN, —NO 2 , —NR m R m , —OR m , —C(═O)OR m , and —OC(═O)R m ; or R 5 and R 2 taken together with the atoms to which they are attached form a heterocyclyl;

each R a is independently selected from H, C 1-6 alkyl, C 2-6 alkenyl, C 2-6 alkynyl, carbocyclyl, and heterocyclyl, wherein each C 1-6 alkyl, C 2-6 alkenyl, C 2-6 alkynyl, carbocyclyl, and heterocyclyl is optionally substituted with one or more groups R x ;

each R f is independently selected from H, C 1-3 alkyl, trifluoromethyl, 3-6 membered carbocyclyl, and 3-6 membered heterocyclyl; or two R f groups together with the nitrogen to which they are attached form a 3-6 membered heterocycle;

each R g is independently selected from H, C 1-6 alkyl, C 2-6 alkenyl, C 2-6 alkynyl, C 3-8 carbocyclyl, and heterocyclyl, wherein each C 1-6 alkyl, C 2-6 alkenyl, C 2-6 alkynyl, C 3-8 carbocyclyl, and heterocyclyl is optionally substituted with one or more groups R x ; or two R g groups together with the nitrogen to which they are attached form a 3-6 membered heterocycle;

each R m is independently selected from H, C 1-6 alkyl, C 2-6 alkenyl, C 2-6 alkynyl, C 1-6 haloalkyl, carbocyclyl, C 1-6 alkanoyl, phenyl, and benzyl, wherein any C 1-6 alkyl, C 2-6 alkenyl, C 2-6 alkynyl, C 1-6 haloalkyl, carbocyclyl, C 1-6 alkanoyl, phenyl, or benzyl is optionally substituted with one or more groups independently selected from halo, —CN, —NO 2 , —NR y R z , and —OR w ; or two R m groups together with the nitrogen to which they are attached form a 3-6 membered heterocycle;

each R v is independently hydrogen, C 1-6 alkyl, C 2-6 alkenyl, C 2-6 alkynyl, carbocyclyl, and heterocyclyl, wherein each C 1-6 alkyl, C 2-6 alkenyl, C 2-6 alkynyl, carbocyclyl, and heterocyclyl is optionally substituted with one or more groups independently selected from oxo, halo, amino, hydroxyl, and C 1 -C 6 alkyl that is optionally substituted with one or more groups independently selected from oxo and halo; or two R v are taken together with the nitrogen to which they are attached to form a heterocyclyl that is optionally substituted with one or more groups independently selected from oxo, halo and C 1-3 alkyl that is optionally substituted with one or more groups independently selected from oxo and halo;

each R w is independently selected from H, C 1-4 alkyl, C 1-4 alkanoyl, phenyl, benzyl, and phenethyl;

each R x is independently selected from oxo, C 1-6 alkyl, C 2-6 alkenyl, C 2-6 alkynyl, C 1-6 haloalkyl, carbocyclyl, —F, —Cl, —Br, —I, —NO 2 , —N(R v ) 2 , —CN, —C(O)—N(R v ) 2 , —S(O)—N(R v ) 2 , —S(O) 2 —N(R v ) 2 , —O—R v , —S—R v , —O—C(O)—R v , —O—C(O)—O—R v , —C(O)—R v , —C(O)—O—R v , —S(O)—R v , —S(O) 2 —R v , —O—C(O)—N(R v ) 2 , —N(R v )—C(O)—OR v , —N(R v )—C(O)—N(R v ) 2 , —S(O) 2 —N(R v ) 2 , —N(R v )—C(O)—R v , —N(R v )—S(O)—R v , —N(R v )—S(O) 2 —R v , —N(R v )—S(O)—N(R v ) 2 , and —N(R v )—S(O) 2 —N(R v ) 2 , wherein any C 1-6 alkyl, C 2-6 alkenyl, C 2-6 alkynyl, C 1-6 haloalkyl and carbocyclyl is optionally substituted with one or more groups independently selected from oxo, halo, —NO 2 , —N(R v ) 2 , —CN,

—C(O)—N(R v ) 2 , —S(O)—N(R v ) 2 , —S(O) 2 —N(R v ) 2 , —O—R v , —S—R v , —O—C(O)—R v , —C(O)—R v , —C(O)—O—R v , —S(O)—R v , —S(O) 2 —R v , —C(O)—N(R v ) 2 , —S(O) 2 —N(R v ) 2 , —N(R v )—C(O)—R v , —N(R v )—S(O)—R v —N(R v )—S(O) 2 —R v and C 1-6 alkyl that is optionally substituted with one or more groups independently selected from oxo and halo; and

each R y and R z is independently selected from H, C 1-4 alkyl, C 1-4 alkanoyl, C 1-4 alkoxycarbonyl, phenyl, benzyl, and phenethyl, or R y and R z together with the nitrogen to which they are attached form a heterocyclyl; or a salt thereof

wherein -A-R 4 taken together is selected from:

3. A compound selected from:

and pharmaceutically acceptable salts thereof.

Assignments (2)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Dec 8, 2015
From: LIANG, JUN; MAGNUSON, STEVEN R.; TSUI, VICKIE HSIAO-WEI; ZHANG, BIRONG
To: GENENTECH, INC.
Reel/Frame 037233/0460 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Dec 8, 2015
From: ALBRECHT, BRIAN K.; BELLON, STEVEN F.; GEHLING, VICTOR S.; HARMANGE, JEAN-CHRISTOPHE; LEBLANC, YVES
To: CONSTELLATION PHARMACEUTICALS, INC.
Reel/Frame 037233/0880 →
Continuity (2)
Provisional Application 61874310 · Sep 5, 2013
Related Publication 20150065522A1 · Mar 5, 2015