Delivery of agents using interfering nanoparticles
View Patent ↗Provided are compositions and methods for delivery of therapeutic agents, such as chemically stabilized antisense oligonucleotides useful in RNA silencing. The compositions include interfering nanoparticles (iNOPs) associated with one or more agents. Several functional iNOP derivatives are provided which allow for targeted delivery of agents to specific cell types as well as exhibiting reduced cellular toxicity.
1. A composition comprising a nanotransporter interfering nanoparticle-7 (iNOP-7) having a polylysine dendrimer generation 4 (LDG4) core conjugated to a lipid functional group having the following structure:
wherein the iNOP-7 is functionalized with any of functional surface groups A-I by being conjugated to iNOP-7 through an amide bond formed via an NH 2 group of the iNOP7:
wherein A forms iNOP-7E; B foul's iNOP-7LE; C foul's iNOP-7DS; D forms iNOP-7His; E-forms iNOP-7Bio; F forms iNOP-7AD; G forms iNOP-7PEG; H forms iNOP-7A; and I forms iNOP-7Lac.
2. The composition of claim 1 , wherein the iNOP-7 is associated with a nucleic acid molecule or pharmaceutical agent.
3. The composition of claim 2 , wherein the iNOP-7 is associated with a nucleic acid molecule.
4. The composition of claim 3 , wherein the nucleic acid molecule is an antisense oligonucleotide.
5. The composition of claim 4 , wherein the antisense oligonucleotide is RNA.
6. The composition of claim 5 , wherein the RNA is chemically modified.
7. The composition of claim 6 , wherein the chemical modification comprises a 2′-O—F, 2′-Ome, 2′MOE, 2′-H, 2′-amino, 4-thioU or 6-thioG modification of one or more nucleotides, introduction of one or more phosphorothioate linkages, introduction of one or more locked nucleotides, or a combination thereof.
8. The composition of claim 5 , wherein the RNA is selected from the group consisting of microRNA mimic, anti-microRNA, dsRNA, siRNA, stRNA, or shRNA.
9. The composition of claim 8 , wherein the RNA is anti-microRNA, microRNA mimic, dsRNA or siRNA.
10. The composition of claim 9 , wherein an antisense strand, a sense strand, or both, of the RNA is chemically modified.
11. The composition of claim 10 , wherein the chemical modification comprises a 2′-O—F, 2′-Ome, 2′MOE, 2′-H, 2′-amino, 4-thioU or 6-thioG modification of one or more nucleotides, introduction of one or more phosphorothioate linkages, introduction of one or more locked nucleotides, or a combination thereof.
12. The composition of claim 5 , wherein the antisense oligonucleotide is about 7-36 nucleotides in length.